- Catalog number
- FEK0541
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1.The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12. The standard product used in this kit is recombinant mouse VEGF164, a 42KDa dimer.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 34 35 37 36 2.29 6.3% Sample 2 179 204 186 197 191 9.65 5% Sample 3 538 463 494 471 491 29.15 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 34 35 37 36 2.29 6.3% Sample 1 40 34 35 37 36 2.29 6.3% Sample 2 179 204 186 197 191 9.65 5% Sample 2 179 204 186 197 191 9.65 5% Sample 3 538 463 494 471 491 29.15 5.9% Sample 3 538 463 494 471 491 29.15 5.9%
- Synonyms
- Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; Vegfa; Vegf
- Gene Name
- Vegfa
- Gene ID
- 22339
- UniProt
- Q00731
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A27-R190
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression of VEGF continues in kidney glomeruli and choroid plexus in adults.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 179 538 40 171 574 Standard deviation 1.92 9.48 36.58 2.6 9.57 48.79 CV (%) 4.8% 5.3% 6.8% 6.5% 5.6% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 179 538 40 171 574 Standard deviation 1.92 9.48 36.58 2.6 9.57 48.79 CV (%) 4.8% 5.3% 6.8% 6.5% 5.6% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 40 179 538 40 171 574 Mean (pg/ml) 40 179 538 40 171 574 Standard deviation 1.92 9.48 36.58 2.6 9.57 48.79 Standard deviation 1.92 9.48 36.58 2.6 9.57 48.79 CV (%) 4.8% 5.3% 6.8% 6.5% 5.6% 8.5% CV (%) 4.8% 5.3% 6.8% 6.5% 5.6% 8.5%
- Sensitivity
- <2 pg/mL
- Function
- Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity) . .
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Calculated Molecular Weight
- 25283 MW
- Specificity
- Natural and recombinant Mouse Vegfa
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Vascular endothelial growth factor A
- Subcellular Location
- Isoform VEGF-1: Secreted.
- Sequence Similarities
- Belongs to the PDGF/VEGF growth factor family.
- Protein Name
- Vascular endothelial growth factor A
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.096 0.178 0.238 0.345 0.554 0.669 1.577 2.029 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.096 0.178 0.238 0.345 0.554 0.669 1.577 2.029 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.096 0.178 0.238 0.345 0.554 0.669 1.577 2.029 O.D. 0.096 0.178 0.238 0.345 0.554 0.669 1.577 2.029
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
