Human IL-15/Interleukin-15 PicoKine® Quick ELISA Kit

About this product

Human IL-15 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests) . Quantitate Human IL15 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0426

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin (IL) -15 is a cytokine with the ability to stimulate the proliferation activity of Th1 and/or Th2 lymphocytes. IL-15 is a novel cytokine whose effects on T-cell activation and proliferation are similar to those of interleukin-2 (IL-2), presumably because IL-15 utilizes the beta and gamma chains of the IL-2 receptor. IL-15 can play a role in the initiation and outcome of acute and chronic rejection. Anti-IL-15 therapy in combination with classic immunosuppression therapy might thus be beneficial in the prevention of acute, and especially chronic, allograft rejection. The human IL15 gene is mapped to human chromosome 4q31 by fluorescence in situ hybridization.1 The standard product used in this kit is recombinant human IL-15, consisting of 114 amino acids with the molecular mass of 12.9KDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 37 37 35 36 1 2.7% Sample 2 140 151 160 164 153 9.22 6% Sample 3 427 395 364 407 398 22.84 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 37 37 35 36 1 2.7% Sample 1 35 37 37 35 36 1 2.7% Sample 2 140 151 160 164 153 9.22 6% Sample 2 140 151 160 164 153 9.22 6% Sample 3 427 395 364 407 398 22.84 5.7% Sample 3 427 395 364 407 398 22.84 5.7%

Synonyms

Interleukin-15; IL-15; IL15

Gene Name

IL15

Gene ID

3600

UniProt

P40933

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N49-S162

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15- S21AA is preferentially expressed in tissues such as testis and thymus.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 140 427 32 143 390 Standard deviation 2.34 6.16 19.64 2.52 8.72 23.79 CV (%) 6.7% 4.4% 4.6% 7.9% 6.1% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 140 427 32 143 390 Standard deviation 2.34 6.16 19.64 2.52 8.72 23.79 CV (%) 6.7% 4.4% 4.6% 7.9% 6.1% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 140 427 32 143 390 Mean (pg/ml) 35 140 427 32 143 390 Standard deviation 2.34 6.16 19.64 2.52 8.72 23.79 Standard deviation 2.34 6.16 19.64 2.52 8.72 23.79 CV (%) 6.7% 4.4% 4.6% 7.9% 6.1% 6.1% CV (%) 6.7% 4.4% 4.6% 7.9% 6.1% 6.1%

Sensitivity

<3 pg/mL

Function

Cytokine that stimulates the proliferation of T- lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.

Components

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

18086 MW

Specificity

Natural and recombinant human IL-15

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-15

Subcellular Location

Isoform IL15-S48AA: Secreted.

Sequence Similarities

Belongs to the IL-15/IL-21 family.

Protein Name

Interleukin-15

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.010 0.112 0.180 0.283 0.668 1.056 1.885 2.523 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.010 0.112 0.180 0.283 0.668 1.056 1.885 2.523 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.010 0.112 0.180 0.283 0.668 1.056 1.885 2.523 O.D. 0.010 0.112 0.180 0.283 0.668 1.056 1.885 2.523

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.