- Catalog number
- FEK0749
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Decorin is a proteoglycan on average 90 - 140 kilodaltons (kD) in size.It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS) .Using a genomic clone as the labeled probe and in situ hybridization of human metaphase chromosomes, decorin gene was mapped to the discrete region of human chromosome 12q23. Decorin is capable of suppressing the growth of various tumor cell lines when expressed ectopically.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 51 50 59 55 4.52 8.2% Sample 2 247 279 302 287 278 20.1 7.2% Sample 3 1001 962 922 976 965 28.61 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 51 50 59 55 4.52 8.2% Sample 1 60 51 50 59 55 4.52 8.2% Sample 2 247 279 302 287 278 20.1 7.2% Sample 2 247 279 302 287 278 20.1 7.2% Sample 3 1001 962 922 976 965 28.61 2.9% Sample 3 1001 962 922 976 965 28.61 2.9%
- Synonyms
- Decorin; Bone proteoglycan II; PG-S2; PG40; DCN; SLRR1B
- Gene Name
- DCN
- Gene ID
- 1634
- UniProt
- P07585
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: D31-K359
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 247 1001 56 270 1067 Standard deviation 4.56 16.54 70.07 4.31 20.25 88.56 CV (%) 7.6% 6.7% 7% 7.7% 7.5% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 247 1001 56 270 1067 Standard deviation 4.56 16.54 70.07 4.31 20.25 88.56 CV (%) 7.6% 6.7% 7% 7.7% 7.5% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 60 247 1001 56 270 1067 Mean (pg/ml) 60 247 1001 56 270 1067 Standard deviation 4.56 16.54 70.07 4.31 20.25 88.56 Standard deviation 4.56 16.54 70.07 4.31 20.25 88.56 CV (%) 7.6% 6.7% 7% 7.7% 7.5% 8.3% CV (%) 7.6% 6.7% 7% 7.7% 7.5% 8.3%
- Sensitivity
- <10 pg/mL
- Function
- May affect the rate of fibrils formation.
- Components
- Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 39747 MW
- Specificity
- Natural and recombinant human Decorin
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Decorin
- Subcellular Location
- Secreted, extracellular space, extracellular matrix.
- Sequence Similarities
- Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
- Protein Name
- Decorin
- Sample Data
- Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 O.D. 0.003 0.067 0.111 0.218 0.407 0.82 1.339 2.091 Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 O.D. 0.003 0.067 0.111 0.218 0.407 0.82 1.339 2.091 Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 O.D. 0.003 0.067 0.111 0.218 0.407 0.82 1.339 2.091 O.D. 0.003 0.067 0.111 0.218 0.407 0.82 1.339 2.091
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
