- Catalog number
- FEK0644
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-beta (TGFB) receptor complex and binds TGFB1 with high affinity.1 CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons.2 The protein consists of a homodimer of 180 kDA with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation.3 Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggested a persisting postmitotic activation in an advanced stage of neovascular tissue.4 The standard product used in this kit is extracellular part of recombinant human CD105, from E26 to G586. As a result of glycosylation, the molecular mass is 75-85KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 312 285 280 306 295 13.53 4.5% Sample 2 1384 1301 1412 1238 1333 68.71 5.1% Sample 3 4821 5271 4779 5474 5086 295.48 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 312 285 280 306 295 13.53 4.5% Sample 1 312 285 280 306 295 13.53 4.5% Sample 2 1384 1301 1412 1238 1333 68.71 5.1% Sample 2 1384 1301 1412 1238 1333 68.71 5.1% Sample 3 4821 5271 4779 5474 5086 295.48 5.8% Sample 3 4821 5271 4779 5474 5086 295.48 5.8%
- Synonyms
- Endoglin; CD105; ENG; END
- Gene Name
- ENG
- Gene ID
- 2022
- UniProt
- P17813
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: E26-G586
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Endoglin is restricted to endothelial cells in all tissues except bone marrow.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 312 1384 4821 316 1275 4955 Standard deviation 16.22 74.73 318.18 17.38 72.67 386.49 CV (%) 5.2% 5.4% 6.6% 5.5% 5.7% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 312 1384 4821 316 1275 4955 Standard deviation 16.22 74.73 318.18 17.38 72.67 386.49 CV (%) 5.2% 5.4% 6.6% 5.5% 5.7% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 312 1384 4821 316 1275 4955 Mean (pg/ml) 312 1384 4821 316 1275 4955 Standard deviation 16.22 74.73 318.18 17.38 72.67 386.49 Standard deviation 16.22 74.73 318.18 17.38 72.67 386.49 CV (%) 5.2% 5.4% 6.6% 5.5% 5.7% 7.8% CV (%) 5.2% 5.4% 6.6% 5.5% 5.7% 7.8%
- Sensitivity
- <15 pg/mL
- Function
- Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF- beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3. .
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Calculated Molecular Weight
- 70578 MW
- Specificity
- Natural and recombinant human CD105
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Endoglin
- Subcellular Location
- Membrane; Single-pass type I membrane protein.
- Protein Name
- Endoglin
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.102 0.258 0.471 0.766 1.312 1.658 2.022 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.102 0.258 0.471 0.766 1.312 1.658 2.022 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.102 0.258 0.471 0.766 1.312 1.658 2.022 O.D. 0.011 0.102 0.258 0.471 0.766 1.312 1.658 2.022
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
