- Catalog number
- FEK1208
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Amyloid P component, serum (SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called amyloid. It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3608 4012 4067 4138 3956 205.96 5.2% Sample 2 12778 11342 12753 12065 12234 589.38 4.8% Sample 3 47613 50001 47706 49348 48667 1034.13 2.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3608 4012 4067 4138 3956 205.96 5.2% Sample 1 3608 4012 4067 4138 3956 205.96 5.2% Sample 2 12778 11342 12753 12065 12234 589.38 4.8% Sample 2 12778 11342 12753 12065 12234 589.38 4.8% Sample 3 47613 50001 47706 49348 48667 1034.13 2.1% Sample 3 47613 50001 47706 49348 48667 1034.13 2.1%
- Synonyms
- Serum amyloid P-component; SAP; Apcs; Ptx2, Sap
- Gene Name
- Apcs
- Gene ID
- 20219
- UniProt
- P12246
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q21-D224
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 100 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 1.56 ng/mL - 100 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3608 12778 47613 3726 13449 43062 Standard deviation 144.32 715.56 2809.16 176.12 981.77 2626.78 CV (%) 4% 5.6% 5.9% 4.7% 7.3% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3608 12778 47613 3726 13449 43062 Standard deviation 144.32 715.56 2809.16 176.12 981.77 2626.78 CV (%) 4% 5.6% 5.9% 4.7% 7.3% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 3608 12778 47613 3726 13449 43062 Mean (pg/ml) 3608 12778 47613 3726 13449 43062 Standard deviation 144.32 715.56 2809.16 176.12 981.77 2626.78 Standard deviation 144.32 715.56 2809.16 176.12 981.77 2626.78 CV (%) 4% 5.6% 5.9% 4.7% 7.3% 6.1% CV (%) 4% 5.6% 5.9% 4.7% 7.3% 6.1%
- Sensitivity
- <50 pg/mL
- Components
- Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 26247 MW
- Specificity
- Natural and recombinant mouse SAP
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Serum amyloid P-component
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the pentaxin family.
- Protein Name
- Serum amyloid P-component
- Sample Data
- Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.002 0.065 0.127 0.246 0.436 0.787 1.246 1.831 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.002 0.065 0.127 0.246 0.436 0.787 1.246 1.831 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.002 0.065 0.127 0.246 0.436 0.787 1.246 1.831 O.D. 0.002 0.065 0.127 0.246 0.436 0.787 1.246 1.831
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
