- Catalog number
- FEK0504
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a homing receptor for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 190 188 188 191 189 1.29 0.6% Sample 2 621 646 683 628 644 24.02 3.7% Sample 3 1771 1866 1906 1738 1820 68.25 3.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 190 188 188 191 189 1.29 0.6% Sample 1 190 188 188 191 189 1.29 0.6% Sample 2 621 646 683 628 644 24.02 3.7% Sample 2 621 646 683 628 644 24.02 3.7% Sample 3 1771 1866 1906 1738 1820 68.25 3.7% Sample 3 1771 1866 1906 1738 1820 68.25 3.7%
- Synonyms
- L-selectin ; Sell
- Gene Name
- Sell
- Gene ID
- 20343
- UniProt
- Q3TCF3
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: W39-N332
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 190 621 1771 204 669 1831 Standard deviation 9.5 32.29 138.13 11.01 34.78 157.46 CV (%) 5% 5.2% 7.8% 5.4% 5.2% 8.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 190 621 1771 204 669 1831 Standard deviation 9.5 32.29 138.13 11.01 34.78 157.46 CV (%) 5% 5.2% 7.8% 5.4% 5.2% 8.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 190 621 1771 204 669 1831 Mean (pg/ml) 190 621 1771 204 669 1831 Standard deviation 9.5 32.29 138.13 11.01 34.78 157.46 Standard deviation 9.5 32.29 138.13 11.01 34.78 157.46 CV (%) 5% 5.2% 7.8% 5.4% 5.2% 8.6% CV (%) 5% 5.2% 7.8% 5.4% 5.2% 8.6%
- Sensitivity
- <5 pg/mL
- Components
- Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 38463 MW
- Specificity
- Natural and recombinant mouse sL-Selectin
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Selectin L
- Protein Name
- L-selectin
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.035 0.074 0.129 0.311 0.527 1.177 1.804 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.035 0.074 0.129 0.311 0.527 1.177 1.804 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.035 0.074 0.129 0.311 0.527 1.177 1.804 O.D. 0.002 0.035 0.074 0.129 0.311 0.527 1.177 1.804
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
