- Catalog number
- FEK0400
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 140 116 139 115 127 12.01 9.4% Sample 2 719 735 616 709 694 46.4 6.6% Sample 3 2353 2484 2499 2400 2434 60.08 2.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 140 116 139 115 127 12.01 9.4% Sample 1 140 116 139 115 127 12.01 9.4% Sample 2 719 735 616 709 694 46.4 6.6% Sample 2 719 735 616 709 694 46.4 6.6% Sample 3 2353 2484 2499 2400 2434 60.08 2.4% Sample 3 2353 2484 2499 2400 2434 60.08 2.4%
- Synonyms
- Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; CD25; IL2RA
- Gene Name
- IL2RA
- Gene ID
- 3559
- UniProt
- P01589
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E22-C213
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 78 pg/mL - 5,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 140 719 2353 141 785 2310 Standard deviation 6.58 41.7 94.12 7.61 47.1 97.02 CV (%) 4.7% 5.8% 4% 5.4% 6% 4.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 140 719 2353 141 785 2310 Standard deviation 6.58 41.7 94.12 7.61 47.1 97.02 CV (%) 4.7% 5.8% 4% 5.4% 6% 4.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 140 719 2353 141 785 2310 Mean (pg/ml) 140 719 2353 141 785 2310 Standard deviation 6.58 41.7 94.12 7.61 47.1 97.02 Standard deviation 6.58 41.7 94.12 7.61 47.1 97.02 CV (%) 4.7% 5.8% 4% 5.4% 6% 4.2% CV (%) 4.7% 5.8% 4% 5.4% 6% 4.2%
- Sensitivity
- <5 pg/mL
- Function
- Receptor for interleukin-2.
- Components
- Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 30819 MW
- Specificity
- Natural and recombinant human CD25
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin-2 receptor subunit alpha
- Subcellular Location
- Membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Contains 2 Sushi (CCP/SCR) domains.
- Protein Name
- Interleukin-2 receptor subunit alpha
- Sample Data
- Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 O.D. 0.016 0.251 0.435 0.770 1.108 1.479 1.853 2.049 Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 O.D. 0.016 0.251 0.435 0.770 1.108 1.479 1.853 2.049 Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 O.D. 0.016 0.251 0.435 0.770 1.108 1.479 1.853 2.049 O.D. 0.016 0.251 0.435 0.770 1.108 1.479 1.853 2.049
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
