Rat RANK PicoKine® Quick ELISA Kit

About this product

Rat RANK PicoKine® Quick ELISA Kit (120 minutes, 96 Tests) . Quantitate Rat Tnfrsf11a in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1281

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Receptor Activator of Nuclear Factor ?o B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain (residues 234-616), which contain three putative TRAF-binding domains (termed I, II, and III) . RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK. The standard in this kit is recombinant human RANK with the sequence of Q29-G213 aa. It is a dipolymer which compose of two chains, and the molecular weight of each is 48kda.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 209 194 185 217 201 12.49 6.2% Sample 2 514 489 548 539 522 23 4.4% Sample 3 1401 1406 1464 1575 1461 70.05 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 209 194 185 217 201 12.49 6.2% Sample 1 209 194 185 217 201 12.49 6.2% Sample 2 514 489 548 539 522 23 4.4% Sample 2 514 489 548 539 522 23 4.4% Sample 3 1401 1406 1464 1575 1461 70.05 4.7% Sample 3 1401 1406 1464 1575 1461 70.05 4.7%

Synonyms

Protein Tnfrsf11a ; Tnfrsf11a

Gene Name

Tnfrsf11a

Gene ID

498206

UniProt

F1M8Z6

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q30-P213

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants and serum.

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 209 514 1401 226 542 1518 Standard deviation 13.58 34.95 88.16 18.3 42.81 97.15 CV (%) 6.5% 6.8% 7.9% 8.1% 7.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 209 514 1401 226 542 1518 Standard deviation 13.58 34.95 88.16 18.3 42.81 97.15 CV (%) 6.5% 6.8% 7.9% 8.1% 7.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 209 514 1401 226 542 1518 Mean (pg/ml) 209 514 1401 226 542 1518 Standard deviation 13.58 34.95 88.16 18.3 42.81 97.15 Standard deviation 13.58 34.95 88.16 18.3 42.81 97.15 CV (%) 6.5% 6.8% 7.9% 8.1% 7.9% 6.4% CV (%) 6.5% 6.8% 7.9% 8.1% 7.9% 6.4%

Sensitivity

<10 pg/mL

Components

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

67607 MW

Specificity

Natural and recombinant rat RANK

Gene Name Synonym

Tumor necrosis factor receptor superfamily, member 11a, NFKB activator

Protein Name

Tumor necrosis factor receptor superfamily member 11A

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.015 0.193 0.352 0.556 0.885 1.411 1.830 2.023 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.015 0.193 0.352 0.556 0.885 1.411 1.830 2.023 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.015 0.193 0.352 0.556 0.885 1.411 1.830 2.023 O.D. 0.015 0.193 0.352 0.556 0.885 1.411 1.830 2.023

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.