- Catalog number
- FEK0309
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF) . BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP) . BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 58 63 68 60 4.32 7.2% Sample 2 190 202 221 232 211 14.55 6.9% Sample 3 932 889 870 804 871 55.74 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 58 63 68 60 4.32 7.2% Sample 1 54 58 63 68 60 4.32 7.2% Sample 2 190 202 221 232 211 14.55 6.9% Sample 2 190 202 221 232 211 14.55 6.9% Sample 3 932 889 870 804 871 55.74 6.4% Sample 3 932 889 870 804 871 55.74 6.4%
- Synonyms
- Bdnf|Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin
- Gene Name
- CST3
- Gene ID
- 12064
- UniProt
- P21237
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: H129-R247
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Monoclonal antibody from mouse
- Applications
- ELISA
- Field of Research
- Atherosclerosis, Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate)
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 54 190 923 64 214 926 Standard deviation 3.99 13.87 49.84 5.5 19.47 59.26 CV (%) 7.4% 7.3% 5.4% 8.6% 9.1% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 54 190 923 64 214 926 Standard deviation 3.99 13.87 49.84 5.5 19.47 59.26 CV (%) 7.4% 7.3% 5.4% 8.6% 9.1% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 54 190 923 64 214 926 Mean (pg/ml) 54 190 923 64 214 926 Standard deviation 3.99 13.87 49.84 5.5 19.47 59.26 Standard deviation 3.99 13.87 49.84 5.5 19.47 59.26 CV (%) 7.4% 7.3% 5.4% 8.6% 9.1% 6.4% CV (%) 7.4% 7.3% 5.4% 8.6% 9.1% 6.4%
- Sensitivity
- <15 pg/mL
- Components
- Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Specificity
- Natural and recombinant Mouse Bdnf
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Brain derived neurotrophic factor
- Protein Name
- Brain-derived neurotrophic factor
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.014 0.076 0.120 0.255 0.485 1.020 1.455 2.001 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.014 0.076 0.120 0.255 0.485 1.020 1.455 2.001 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.014 0.076 0.120 0.255 0.485 1.020 1.455 2.001 O.D. 0.014 0.076 0.120 0.255 0.485 1.020 1.455 2.001
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
