- Catalog number
- FEK0527
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte- macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 20 22 18 19 19 0.89 4.7% Sample 2 55 58 64 57 58 3.65 6.3% Sample 3 314 297 303 287 300 8.1 2.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 20 22 18 19 19 0.89 4.7% Sample 1 20 22 18 19 19 0.89 4.7% Sample 2 55 58 64 57 58 3.65 6.3% Sample 2 55 58 64 57 58 3.65 6.3% Sample 3 314 297 303 287 300 8.1 2.7% Sample 3 314 297 303 287 300 8.1 2.7%
- Synonyms
- TNF-alpha|APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2) ; tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
- Gene Name
- CST3
- Gene ID
- 21926
- UniProt
- P06804
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: L80-L235
- Capture Antibody
- Monoclonal antibody from hamster
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Atherosclerosis, Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL (cell culture supernatants), 7.8 pg/mL - 500 pg/mL (mouse serum, plasma)
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 20 55 314 18 63 350 Standard deviation 1.04 3.74 23.55 1.06 4.59 28.7 CV (%) 5.2% 6.8% 7.5% 5.9% 7.3% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 20 55 314 18 63 350 Standard deviation 1.04 3.74 23.55 1.06 4.59 28.7 CV (%) 5.2% 6.8% 7.5% 5.9% 7.3% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 20 55 314 18 63 350 Mean (pg/ml) 20 55 314 18 63 350 Standard deviation 1.04 3.74 23.55 1.06 4.59 28.7 Standard deviation 1.04 3.74 23.55 1.06 4.59 28.7 CV (%) 5.2% 6.8% 7.5% 5.9% 7.3% 8.2% CV (%) 5.2% 6.8% 7.5% 5.9% 7.3% 8.2%
- Sensitivity
- <1 pg/mL
- Components
- Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Specificity
- Natural and recombinant Mouse Tnf
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Tumor necrosis factor
- Protein Name
- Tumor necrosis factor
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.160 0.232 0.311 0.406 0.654 1.065 1.673 2.430 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.160 0.232 0.311 0.406 0.654 1.065 1.673 2.430 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.160 0.232 0.311 0.406 0.654 1.065 1.673 2.430 O.D. 0.160 0.232 0.311 0.406 0.654 1.065 1.673 2.430
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
