- Catalog number
- FEK0672
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 191 201 226 217 209 11.48 5.5% Sample 2 1701 1814 1695 1596 1702 76.57 4.5% Sample 3 5274 5267 4806 4977 5081 157.51 3.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 191 201 226 217 209 11.48 5.5% Sample 1 191 201 226 217 209 11.48 5.5% Sample 2 1701 1814 1695 1596 1702 76.57 4.5% Sample 2 1701 1814 1695 1596 1702 76.57 4.5% Sample 3 5274 5267 4806 4977 5081 157.51 3.1% Sample 3 5274 5267 4806 4977 5081 157.51 3.1%
- Gene Name
- CST3
- Gene ID
- 1509
- UniProt
- P07339
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L21-L412
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 191 1701 5274 179 1699 5098 Standard deviation 13.56 100.36 358.63 13.43 108.74 433.33 CV (%) 7.1% 5.9% 6.8% 7.5% 6.4% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 191 1701 5274 179 1699 5098 Standard deviation 13.56 100.36 358.63 13.43 108.74 433.33 CV (%) 7.1% 5.9% 6.8% 7.5% 6.4% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 191 1701 5274 179 1699 5098 Mean (pg/ml) 191 1701 5274 179 1699 5098 Standard deviation 13.56 100.36 358.63 13.43 108.74 433.33 Standard deviation 13.56 100.36 358.63 13.43 108.74 433.33 CV (%) 7.1% 5.9% 6.8% 7.5% 6.4% 8.5% CV (%) 7.1% 5.9% 6.8% 7.5% 6.4% 8.5%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Specificity
- Natural and recombinant Human CTSD
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Cathepsin D
- Protein Name
- Cathepsin D
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.123 0.255 0.462 0.775 1.343 1.769 2.094 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.123 0.255 0.462 0.775 1.343 1.769 2.094 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.052 0.123 0.255 0.462 0.775 1.343 1.769 2.094 O.D. 0.052 0.123 0.255 0.462 0.775 1.343 1.769 2.094
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
