Human HAI-2/SPINT2 PicoKine® Quick ELISA Kit

About this product

Human HAI-2/SPINT2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human SPINT2 in cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0773

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Kunitz-type protease inhibitor 2 is an enzyme inhibitor that in humans is encoded by the SPINT2 gene. This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. And this gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 51 45 47 51 2.22 4.4% Sample 2 211 221 223 199 214 8.33 3.9% Sample 3 923 961 826 917 907 53.5 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 51 45 47 51 2.22 4.4% Sample 1 59 51 45 47 51 2.22 4.4% Sample 2 211 221 223 199 214 8.33 3.9% Sample 2 211 221 223 199 214 8.33 3.9% Sample 3 923 961 826 917 907 53.5 5.9% Sample 3 923 961 826 917 907 53.5 5.9%

Gene Name

CST3

Gene ID

10653

UniProt

O43291

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-K197

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 211 923 61 224 890 Standard deviation 3.07 10.97 61.84 3.54 12.32 62.3 CV (%) 5.2% 5.2% 6.7% 5.8% 5.5% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 211 923 61 224 890 Standard deviation 3.07 10.97 61.84 3.54 12.32 62.3 CV (%) 5.2% 5.2% 6.7% 5.8% 5.5% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 59 211 923 61 224 890 Mean (pg/ml) 59 211 923 61 224 890 Standard deviation 3.07 10.97 61.84 3.54 12.32 62.3 Standard deviation 3.07 10.97 61.84 3.54 12.32 62.3 CV (%) 5.2% 5.2% 6.7% 5.8% 5.5% 7% CV (%) 5.2% 5.2% 6.7% 5.8% 5.5% 7%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human SPINT2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Serine peptidase inhibitor, Kunitz type, 2

Protein Name

Kunitz-type protease inhibitor 2

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.096 0.157 0.202 0.298 0.473 0.787 1.322 2.105 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.096 0.157 0.202 0.298 0.473 0.787 1.322 2.105 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.096 0.157 0.202 0.298 0.473 0.787 1.322 2.105 O.D. 0.096 0.157 0.202 0.298 0.473 0.787 1.322 2.105

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.