- Catalog number
- FEK0882
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rat is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the rat Tim1 gene and database analysis, was mapped the HAVCR1 gene to 5q33.2.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 93 105 102 97 5.34 5.5% Sample 2 289 271 296 302 290 16.79 5.8% Sample 3 921 881 935 899 909 34.54 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 93 105 102 97 5.34 5.5% Sample 1 88 93 105 102 97 5.34 5.5% Sample 2 289 271 296 302 290 16.79 5.8% Sample 2 289 271 296 302 290 16.79 5.8% Sample 3 921 881 935 899 909 34.54 3.8% Sample 3 921 881 935 899 909 34.54 3.8%
- Gene Name
- CST3
- Gene ID
- 286934
- UniProt
- O54947
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S18-V238
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and urine
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 289 921 84 293 985 Standard deviation 3.7 20.52 69.08 3.7 22.56 79.79 CV (%) 4.2% 7.1% 7.5% 4.4% 7.7% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 289 921 84 293 985 Standard deviation 3.7 20.52 69.08 3.7 22.56 79.79 CV (%) 4.2% 7.1% 7.5% 4.4% 7.7% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 88 289 921 84 293 985 Mean (pg/ml) 88 289 921 84 293 985 Standard deviation 3.7 20.52 69.08 3.7 22.56 79.79 Standard deviation 3.7 20.52 69.08 3.7 22.56 79.79 CV (%) 4.2% 7.1% 7.5% 4.4% 7.7% 8.1% CV (%) 4.2% 7.1% 7.5% 4.4% 7.7% 8.1%
- Sensitivity
- <2 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Specificity
- Natural and recombinant Rat Havcr1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Hepatitis A virus cellular receptor 1
- Protein Name
- Hepatitis A virus cellular receptor 1
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.118 0.202 0.263 0.380 0.589 0.977 1.564 2.235 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.118 0.202 0.263 0.380 0.589 0.977 1.564 2.235 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.118 0.202 0.263 0.380 0.589 0.977 1.564 2.235 O.D. 0.118 0.202 0.263 0.380 0.589 0.977 1.564 2.235
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
