- Catalog number
- FEK0539
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis.VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy.VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 77 81 85 82 81 4.96 6.1% Sample 2 280 266 264 251 265 11.94 4.5% Sample 3 953 890 963 998 951 52.31 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 77 81 85 82 81 4.96 6.1% Sample 1 77 81 85 82 81 4.96 6.1% Sample 2 280 266 264 251 265 11.94 4.5% Sample 2 280 266 264 251 265 11.94 4.5% Sample 3 953 890 963 998 951 52.31 5.5% Sample 3 953 890 963 998 951 52.31 5.5%
- Gene Name
- CST3
- Gene ID
- 7422
- UniProt
- P15692
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A27-R191
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL (human serum, plasma), 15.6 pg/mL - 1,000 pg/mL (cell culture supernates)
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 77 280 953 72 263 988 Standard deviation 7.32 21.84 89.58 5.54 24.72 73.11 CV (%) 9.5% 7.8% 9.4% 7.7% 9.4% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 77 280 953 72 263 988 Standard deviation 7.32 21.84 89.58 5.54 24.72 73.11 CV (%) 9.5% 7.8% 9.4% 7.7% 9.4% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 77 280 953 72 263 988 Mean (pg/ml) 77 280 953 72 263 988 Standard deviation 7.32 21.84 89.58 5.54 24.72 73.11 Standard deviation 7.32 21.84 89.58 5.54 24.72 73.11 CV (%) 9.5% 7.8% 9.4% 7.7% 9.4% 7.4% CV (%) 9.5% 7.8% 9.4% 7.7% 9.4% 7.4%
- Sensitivity
- <1 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Specificity
- Natural and recombinant Human VEGFA
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Vascular endothelial growth factor A
- Protein Name
- Vascular endothelial growth factor A
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.130 0.338 0.523 0.912 1.480 2.228 2.473 2.536 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.130 0.338 0.523 0.912 1.480 2.228 2.473 2.536 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.130 0.338 0.523 0.912 1.480 2.228 2.473 2.536 O.D. 0.130 0.338 0.523 0.912 1.480 2.228 2.473 2.536
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
