- Catalog number
- FEK0496
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 30 36 29 32 1.65 5.2% Sample 2 212 180 196 206 199 9.73 4.9% Sample3 364 397 405 374 385 6.93 1.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 30 36 29 32 1.65 5.2% Sample 1 32 30 36 29 32 1.65 5.2% Sample 2 212 180 196 206 199 9.73 4.9% Sample 2 212 180 196 206 199 9.73 4.9% Sample3 364 397 405 374 385 6.93 1.8% Sample3 364 397 405 374 385 6.93 1.8%
- Gene Name
- CST3
- Gene ID
- 81780
- UniProt
- P50231
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: S24-S91
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants. serum and plasma (heparin, EDTA, citrate)
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 212 364 36 199 351 Standard deviation 2.02 16.32 20.02 2.74 14.13 25.62 CV (%) 6.3% 7.7% 5.5% 7.6% 7.1% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 212 364 36 199 351 Standard deviation 2.02 16.32 20.02 2.74 14.13 25.62 CV (%) 6.3% 7.7% 5.5% 7.6% 7.1% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 32 212 364 36 199 351 Mean (pg/ml) 32 212 364 36 199 351 Standard deviation 2.02 16.32 20.02 2.74 14.13 25.62 Standard deviation 2.02 16.32 20.02 2.74 14.13 25.62 CV (%) 6.3% 7.7% 5.5% 7.6% 7.1% 7.3% CV (%) 6.3% 7.7% 5.5% 7.6% 7.1% 7.3%
- Sensitivity
- <1 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Specificity
- Natural and recombinant Rat Ccl5
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Chemokine (C-C motif) ligand 5
- Protein Name
- C-C motif chemokine 5
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.016 0.164 0.255 0.450 0.831 1.445 2.087 2.262 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.016 0.164 0.255 0.450 0.831 1.445 2.087 2.262 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.016 0.164 0.255 0.450 0.831 1.445 2.087 2.262 O.D. 0.016 0.164 0.255 0.450 0.831 1.445 2.087 2.262
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
