Human MMP-3 PicoKine® Quick ELISA Kit

About this product

Human MMP-3 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human MMP3 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0461

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Fibronectin

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 288 301 305 267 290 18.29 6.3% Sample 2 1542 1548 1686 1602 1595 52.62 3.3% Sample 3 4697 3934 4512 4539 4421 256.39 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 288 301 305 267 290 18.29 6.3% Sample 1 288 301 305 267 290 18.29 6.3% Sample 2 1542 1548 1686 1602 1595 52.62 3.3% Sample 2 1542 1548 1686 1602 1595 52.62 3.3% Sample 3 4697 3934 4512 4539 4421 256.39 5.8% Sample 3 4697 3934 4512 4539 4421 256.39 5.8%

Gene Name

CST3

Gene ID

4314

UniProt

P08254

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y18-C477

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 288 1542 4697 315 1765 4511 Standard deviation 19.01 98.69 295.91 20.16 118.26 311.26 CV (%) 6.6% 6.4% 6.3% 6.4% 6.7% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 288 1542 4697 315 1765 4511 Standard deviation 19.01 98.69 295.91 20.16 118.26 311.26 CV (%) 6.6% 6.4% 6.3% 6.4% 6.7% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 288 1542 4697 315 1765 4511 Mean (pg/ml) 288 1542 4697 315 1765 4511 Standard deviation 19.01 98.69 295.91 20.16 118.26 311.26 Standard deviation 19.01 98.69 295.91 20.16 118.26 311.26 CV (%) 6.6% 6.4% 6.3% 6.4% 6.7% 6.9% CV (%) 6.6% 6.4% 6.3% 6.4% 6.7% 6.9%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human MMP3

Anticoagulant

Heparin

Gene Name Synonym

Matrix metallopeptidase 3 (stromelysin 1, progelatinase)

Protein Name

Stromelysin-1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.148 0.231 0.392 0.716 1.254 2.061 2.405 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.148 0.231 0.392 0.716 1.254 2.061 2.405 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.148 0.231 0.392 0.716 1.254 2.061 2.405 O.D. 0.042 0.148 0.231 0.392 0.716 1.254 2.061 2.405

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.