- Catalog number
- FEK1257
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Ficolin-1, and also commonly termed M-ficolin is a protein that in humans is encoded by the FCN1 gene. It is mapped to 9q34.3. The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins have a crucial role in defense against pneumococcal infection through the lectin complement pathway. Ficolin 1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1450 1365 1605 1564 1496 97.24 6.5% Sample 2 8641 7641 7712 7952 7987 263.55 3.3% Sample 3 19842 22647 21642 20458 21147 1141.95 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1450 1365 1605 1564 1496 97.24 6.5% Sample 1 1450 1365 1605 1564 1496 97.24 6.5% Sample 2 8641 7641 7712 7952 7987 263.55 3.3% Sample 2 8641 7641 7712 7952 7987 263.55 3.3% Sample 3 19842 22647 21642 20458 21147 1141.95 5.4% Sample 3 19842 22647 21642 20458 21147 1141.95 5.4%
- Gene Name
- CST3
- Gene ID
- 2219
- UniProt
- O00602
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A30-A326
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated
- Standard
- 50 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin)
- Detection Range
- 0.78 ng/mL - 50 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1450 8641 19842 1321 8694 19651 Standard deviation 92.8 475.26 1111.15 93.79 512.95 1061.15 CV (%) 6.4% 5.5% 5.6% 7.1% 5.9% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1450 8641 19842 1321 8694 19651 Standard deviation 92.8 475.26 1111.15 93.79 512.95 1061.15 CV (%) 6.4% 5.5% 5.6% 7.1% 5.9% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1450 8641 19842 1321 8694 19651 Mean (pg/ml) 1450 8641 19842 1321 8694 19651 Standard deviation 92.8 475.26 1111.15 93.79 512.95 1061.15 Standard deviation 92.8 475.26 1111.15 93.79 512.95 1061.15 CV (%) 6.4% 5.5% 5.6% 7.1% 5.9% 5.4% CV (%) 6.4% 5.5% 5.6% 7.1% 5.9% 5.4%
- Sensitivity
- <20 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Specificity
- Natural and recombinant Human FCN1
- Anticoagulant
- Heparin
- Gene Name Synonym
- Ficolin (collagen/fibrinogen domain containing) 1
- Protein Name
- Ficolin-1
- Sample Data
- Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.003 0.177 0.313 0.558 0.926 1.491 1.972 2.357 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.003 0.177 0.313 0.558 0.926 1.491 1.972 2.357 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.003 0.177 0.313 0.558 0.926 1.491 1.972 2.357 O.D. 0.003 0.177 0.313 0.558 0.926 1.491 1.972 2.357
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
