- Catalog number
- FEK0961
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cathepsin S, also known as CTSS, is a protein that in humans is encoded by the CTSS gene. A member of the peptidase C1 family, CTSS gene is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. By fluorescence in situ hybridization (FISH), this gene is mapped to 1q21.3. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.
- Reproducibility
- Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 179 186 175 193 183 6.58 3.6% Sample 2 406 440 371 396 403 24.98 6.2% Sample 3 501 531 479 463 493 25.63 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 179 186 175 193 183 6.58 3.6% Sample 1 179 186 175 193 183 6.58 3.6% Sample 2 406 440 371 396 403 24.98 6.2% Sample 2 406 440 371 396 403 24.98 6.2% Sample 3 501 531 479 463 493 25.63 5.2% Sample 3 501 531 479 463 493 25.63 5.2%
- Gene Name
- CTSS
- Gene ID
- 1520
- UniProt
- P25774
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q17-I331 & S109-I331
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 179 406 1977 190 417 1912 Standard deviation 9.30 19.48 136.41 11.59 27.10 149.13 CV (%) 5.2% 4.8% 6.9% 6.1% 6.5% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 179 406 1977 190 417 1912 Standard deviation 9.30 19.48 136.41 11.59 27.10 149.13 CV (%) 5.2% 4.8% 6.9% 6.1% 6.5% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 179 406 1977 190 417 1912 Mean (pg/ml) 179 406 1977 190 417 1912 Standard deviation 9.30 19.48 136.41 11.59 27.10 149.13 Standard deviation 9.30 19.48 136.41 11.59 27.10 149.13 CV (%) 5.2% 4.8% 6.9% 6.1% 6.5% 7.8% CV (%) 5.2% 4.8% 6.9% 6.1% 6.5% 7.8%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Specificity
- Natural and recombinant Human CTSS
- Anticoagulant
- Heparin or EDTA
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.051 0.192 0.296 0.512 0.884 1.530 2.209 2.316 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.051 0.192 0.296 0.512 0.884 1.530 2.209 2.316 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.051 0.192 0.296 0.512 0.884 1.530 2.209 2.316 O.D. 0.051 0.192 0.296 0.512 0.884 1.530 2.209 2.316
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
