Human CA1/Carbonic anhydrase 1 PicoKine® Quick ELISA Kit

About this product

Human CA1/Carbonic anhydrase 1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CA1/Carbonic anhydrase 1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0680

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Carbonic anhydrase 1 is an enzyme that in humans is encoded by the CA1 gene. It is a member of the Carbonic anhydrase. The CA1 gene is mapped to 8q22. CAI has got about 260 amino acids. This protein is highly expressed in erythrocytes. As catalysts of the reversible hydration of carbon dioxide, CAI participates in a variety of biologic processes like respiration, calcification, acid-base balance etc.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 454 513 503 485 488 17.56 3.6% Sample 2 2602 2853 2812 2793 2765 88.48 3.2% Sample 3 9798 9693 9697 8593 9445 500.58 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 454 513 503 485 488 17.56 3.6% Sample 1 454 513 503 485 488 17.56 3.6% Sample 2 2602 2853 2812 2793 2765 88.48 3.2% Sample 2 2602 2853 2812 2793 2765 88.48 3.2% Sample 3 9798 9693 9697 8593 9445 500.58 5.3% Sample 3 9798 9693 9697 8593 9445 500.58 5.3%

Gene Name

CA1

Gene ID

759

UniProt

P00915

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-F261

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 454 2602 9798 596 2668 9998 Standard deviation 19.06 148.31 627.07 30.39 120.06 559.88 CV (%) 4.2% 5.7% 6.4% 5.1% 4.5% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 454 2602 9798 596 2668 9998 Standard deviation 19.06 148.31 627.07 30.39 120.06 559.88 CV (%) 4.2% 5.7% 6.4% 5.1% 4.5% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 454 2602 9798 596 2668 9998 Mean (pg/ml) 454 2602 9798 596 2668 9998 Standard deviation 19.06 148.31 627.07 30.39 120.06 559.88 Standard deviation 19.06 148.31 627.07 30.39 120.06 559.88 CV (%) 4.2% 5.7% 6.4% 5.1% 4.5% 5.6% CV (%) 4.2% 5.7% 6.4% 5.1% 4.5% 5.6%

Sensitivity

<50 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CA1

Anticoagulant

Heparin, EDTA or citrate

Sample Data

Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.149 0.282 0.395 0.594 0.945 1.467 1.983 2.303 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.149 0.282 0.395 0.594 0.945 1.467 1.983 2.303 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 Concentration (pg/ml) 0 312 625 1,250 2,500 5,000 10,000 20,000 O.D. 0.149 0.282 0.395 0.594 0.945 1.467 1.983 2.303 O.D. 0.149 0.282 0.395 0.594 0.945 1.467 1.983 2.303

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.