Human CCL4/MIP-1 beta PicoKine® Quick ELISA Kit

About this product

Human CCL4/MIP-1 beta PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CCL4/MIP-1 beta in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 4 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0450

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in humans is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. the CCL4 gene was assigned to a slightly more distal location: 17q21-q23 rather than 17q11-q21.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 45 47 40 41 43 1.55 3.8% Sample 2 333 237 173 219 240 18.48 7.7% Sample 3 503 533 481 465 495 26.23 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 45 47 40 41 43 1.55 3.8% Sample 1 45 47 40 41 43 1.55 3.8% Sample 2 333 237 173 219 240 18.48 7.7% Sample 2 333 237 173 219 240 18.48 7.7% Sample 3 503 533 481 465 495 26.23 5.3% Sample 3 503 533 481 465 495 26.23 5.3%

Gene Name

CCL4

Gene ID

6351

UniProt

P13236

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A24-N92

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 45 333 503 39 341 577 Standard deviation 1.93 22.97 39.73 2.41 25.57 50.77 CV (%) 4.3% 6.9% 7.9% 6.2% 7.5% 8.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 45 333 503 39 341 577 Standard deviation 1.93 22.97 39.73 2.41 25.57 50.77 CV (%) 4.3% 6.9% 7.9% 6.2% 7.5% 8.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 45 333 503 39 341 577 Mean (pg/ml) 45 333 503 39 341 577 Standard deviation 1.93 22.97 39.73 2.41 25.57 50.77 Standard deviation 1.93 22.97 39.73 2.41 25.57 50.77 CV (%) 4.3% 6.9% 7.9% 6.2% 7.5% 8.8% CV (%) 4.3% 6.9% 7.9% 6.2% 7.5% 8.8%

Sensitivity

<4 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CCL4

Anticoagulant

Heparin, EDTA or citrate

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.020 0.107 0.182 0.309 0.576 1.050 1.825 2.001 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.020 0.107 0.182 0.309 0.576 1.050 1.825 2.001 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.020 0.107 0.182 0.309 0.576 1.050 1.825 2.001 O.D. 0.020 0.107 0.182 0.309 0.576 1.050 1.825 2.001

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.