Mouse MIP-2/CXCL2 PicoKine® Quick ELISA Kit

About this product

Mouse MIP-2/CXCL2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse MIP-2/CXCL2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 5 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0452

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Macrophage Inflammatory Protein 2 (MIP2), also known as C-X-C motif chemokine 2 (CXCL2), is a small cytokine belonging to the CXC chemokine family. MIP2 forms homotetramers. MIP2 is produced by activated monocytes and neutrophils and expressed at sites of inflammation. MIP2 is chemotactic for polymorphonuclear leukocytes.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 31 40 33 35 2.28 6.5% Sample 2 178 166 181 169 174 3.99 2.3% Sample 3 508 533 443 452 484 21.3 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 31 40 33 35 2.28 6.5% Sample 1 36 31 40 33 35 2.28 6.5% Sample 2 178 166 181 169 174 3.99 2.3% Sample 2 178 166 181 169 174 3.99 2.3% Sample 3 508 533 443 452 484 21.3 4.4% Sample 3 508 533 443 452 484 21.3 4.4%

Gene Name

Cxcl2

Gene ID

20310

UniProt

P10889

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A28-N100

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 178 508 44 167 510 Standard deviation 1.94 12.28 28.45 2.86 9.85 32.13 CV (%) 5.4% 6.9% 5.6% 6.5% 5.9% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 178 508 44 167 510 Standard deviation 1.94 12.28 28.45 2.86 9.85 32.13 CV (%) 5.4% 6.9% 5.6% 6.5% 5.9% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 178 508 44 167 510 Mean (pg/ml) 36 178 508 44 167 510 Standard deviation 1.94 12.28 28.45 2.86 9.85 32.13 Standard deviation 1.94 12.28 28.45 2.86 9.85 32.13 CV (%) 5.4% 6.9% 5.6% 6.5% 5.9% 6.3% CV (%) 5.4% 6.9% 5.6% 6.5% 5.9% 6.3%

Sensitivity

<5 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Mouse Cxcl2

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.027 0.113 0.179 0.297 0.533 0.979 1.710 2.387 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.027 0.113 0.179 0.297 0.533 0.979 1.710 2.387 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.027 0.113 0.179 0.297 0.533 0.979 1.710 2.387 O.D. 0.027 0.113 0.179 0.297 0.533 0.979 1.710 2.387

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.