Human CXCL6/GCP2 PicoKine® Quick ELISA Kit

About this product

Human CXCL6/GCP2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CXCL6/GCP2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK0359

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Chemokine (C-X-C motif) ligand 6 (CXCL6) is a small cytokine belonging to the CXC chemokine family that is also known as granulocyte chemotactic protein 2 (GCP-2) . As its former name suggests, CXCL6 is a chemoattractant for neutrophilic granulocytes. It elicits its chemotactic effects by interacting with the chemokine receptors CXCR1 and CXCR2. The gene for CXCL6 is located on human chromosome 4 in a cluster with other CXC chemokine genes.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 57 54 56 56 1.39 2.5% Sample 2 294 297 303 284 295 8.25 2.8% Sample 3 820 854 836 899 852 30.68 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 57 54 56 56 1.39 2.5% Sample 1 55 57 54 56 56 1.39 2.5% Sample 2 294 297 303 284 295 8.25 2.8% Sample 2 294 297 303 284 295 8.25 2.8% Sample 3 820 854 836 899 852 30.68 3.6% Sample 3 820 854 836 899 852 30.68 3.6%

Gene Name

CXCL6

Gene ID

6372

UniProt

P80162

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V40-N114

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 294 820 47 312 874 Standard deviation 2.26 17.93 36.08 2.3 19.03 48.07 CV (%) 4.1% 6.1% 4.4% 4.9% 6.1% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 294 820 47 312 874 Standard deviation 2.26 17.93 36.08 2.3 19.03 48.07 CV (%) 4.1% 6.1% 4.4% 4.9% 6.1% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 55 294 820 47 312 874 Mean (pg/ml) 55 294 820 47 312 874 Standard deviation 2.26 17.93 36.08 2.3 19.03 48.07 Standard deviation 2.26 17.93 36.08 2.3 19.03 48.07 CV (%) 4.1% 6.1% 4.4% 4.9% 6.1% 5.5% CV (%) 4.1% 6.1% 4.4% 4.9% 6.1% 5.5%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CXCL6

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.011 0.321 0.514 0.927 1.554 2.177 2.375 2.398 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.011 0.321 0.514 0.927 1.554 2.177 2.375 2.398 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.011 0.321 0.514 0.927 1.554 2.177 2.375 2.398 O.D. 0.011 0.321 0.514 0.927 1.554 2.177 2.375 2.398

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.