- Catalog number
- FEK0726
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4) . The PF4 gene was localized on 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
- Reproducibility
- Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 366 303 355 314 335 23.08 6.9% Sample 2 1651 1700 1818 1664 1708 63.21 3.7% Sample 3 4823 4798 4635 4316 4643 190.36 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 366 303 355 314 335 23.08 6.9% Sample 1 366 303 355 314 335 23.08 6.9% Sample 2 1651 1700 1818 1664 1708 63.21 3.7% Sample 2 1651 1700 1818 1664 1708 63.21 3.7% Sample 3 4823 4798 4635 4316 4643 190.36 4.1% Sample 3 4823 4798 4635 4316 4643 190.36 4.1%
- Gene Name
- PF4
- Gene ID
- 5196
- UniProt
- P02776
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: E32-S101
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 366 1651 4823 342 1688 5103 Standard deviation 24.16 80.9 255.62 24.28 97.9 326.59 CV (%) 6.6% 4.9% 5.3% 7.1% 5.8% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 366 1651 4823 342 1688 5103 Standard deviation 24.16 80.9 255.62 24.28 97.9 326.59 CV (%) 6.6% 4.9% 5.3% 7.1% 5.8% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 366 1651 4823 342 1688 5103 Mean (pg/ml) 366 1651 4823 342 1688 5103 Standard deviation 24.16 80.9 255.62 24.28 97.9 326.59 Standard deviation 24.16 80.9 255.62 24.28 97.9 326.59 CV (%) 6.6% 4.9% 5.3% 7.1% 5.8% 6.4% CV (%) 6.6% 4.9% 5.3% 7.1% 5.8% 6.4%
- Sensitivity
- <15 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Specificity
- Natural and recombinant Human PF4
- Anticoagulant
- Heparin or EDTA
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.017 0.149 0.244 0.438 0.875 1.606 2.272 2.492 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.017 0.149 0.244 0.438 0.875 1.606 2.272 2.492 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 Concentration (pg/ml) 0 156 312 625 1,250 2,500 5,000 10,000 O.D. 0.017 0.149 0.244 0.438 0.875 1.606 2.272 2.492 O.D. 0.017 0.149 0.244 0.438 0.875 1.606 2.272 2.492
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
