- Catalog number
- FEK0717
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152 (Cluster of differentiation 152), is a protein receptor that down regulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.
- Reproducibility
- Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 91 95 99 93 3.08 3.3% Sample 2 302 299 240 274 279 13.66 4.9% Sample 3 874 726 864 791 814 42.32 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 91 95 99 93 3.08 3.3% Sample 1 88 91 95 99 93 3.08 3.3% Sample 2 302 299 240 274 279 13.66 4.9% Sample 2 302 299 240 274 279 13.66 4.9% Sample 3 874 726 864 791 814 42.32 5.2% Sample 3 874 726 864 791 814 42.32 5.2%
- Gene Name
- Ctla4
- Gene ID
- 12477
- UniProt
- P09793
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A37-F162
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin)
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 302 874 79 274 826 Standard deviation 4.84 20.84 41.95 5.21 16.17 58.65 CV (%) 5.5% 6.9% 4.8% 6.6% 5.9% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 302 874 79 274 826 Standard deviation 4.84 20.84 41.95 5.21 16.17 58.65 CV (%) 5.5% 6.9% 4.8% 6.6% 5.9% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 88 302 874 79 274 826 Mean (pg/ml) 88 302 874 79 274 826 Standard deviation 4.84 20.84 41.95 5.21 16.17 58.65 Standard deviation 4.84 20.84 41.95 5.21 16.17 58.65 CV (%) 5.5% 6.9% 4.8% 6.6% 5.9% 7.1% CV (%) 5.5% 6.9% 4.8% 6.6% 5.9% 7.1%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Specificity
- Natural and recombinant Mouse Ctla4
- Anticoagulant
- Heparin
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.016 0.129 0.228 0.392 0.726 1.301 1.978 2.589 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.016 0.129 0.228 0.392 0.726 1.301 1.978 2.589 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.016 0.129 0.228 0.392 0.726 1.301 1.978 2.589 O.D. 0.016 0.129 0.228 0.392 0.726 1.301 1.978 2.589
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
