- Catalog number
- FEK1329
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chemerin, also known as RARRES2 or TIG2, is a protein that in humans is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1 (also known as ChemR23) . It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What’s more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.
- Reproducibility
- Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 118 122 103 115 115 6.76 5.9% Sample 2 754 766 687 729 734 27.16 3.7% Sample 3 3266 2789 2969 3381 3101 192.28 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 118 122 103 115 115 6.76 5.9% Sample 1 118 122 103 115 115 6.76 5.9% Sample 2 754 766 687 729 734 27.16 3.7% Sample 2 754 766 687 729 734 27.16 3.7% Sample 3 3266 2789 2969 3381 3101 192.28 6.2% Sample 3 3266 2789 2969 3381 3101 192.28 6.2%
- Gene Name
- RARRES2
- Gene ID
- 5919
- UniProt
- Q99969
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: V17-S163
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 50 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
- Detection Range
- 78 pg/mL - 5,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 118 754 3266 128 779 3354 Standard deviation 6.49 47.5 192.69 6.66 57.65 197.89 CV (%) 5.5% 6.3% 5.9% 5.2% 7.4% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 118 754 3266 128 779 3354 Standard deviation 6.49 47.5 192.69 6.66 57.65 197.89 CV (%) 5.5% 6.3% 5.9% 5.2% 7.4% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 118 754 3266 128 779 3354 Mean (pg/ml) 118 754 3266 128 779 3354 Standard deviation 6.49 47.5 192.69 6.66 57.65 197.89 Standard deviation 6.49 47.5 192.69 6.66 57.65 197.89 CV (%) 5.5% 6.3% 5.9% 5.2% 7.4% 5.9% CV (%) 5.5% 6.3% 5.9% 5.2% 7.4% 5.9%
- Sensitivity
- <20 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Specificity
- Natural and recombinant Human RARRES2
- Anticoagulant
- Heparin or EDTA
- Sample Data
- Concentration (pg/ml) 0 78 156 312 625 1,250 2,500 5,000 O.D. 0.013 0.119 0.195 0.356 0.623 1.012 1.389 1.802 Concentration (pg/ml) 0 78 156 312 625 1,250 2,500 5,000 O.D. 0.013 0.119 0.195 0.356 0.623 1.012 1.389 1.802 Concentration (pg/ml) 0 78 156 312 625 1,250 2,500 5,000 Concentration (pg/ml) 0 78 156 312 625 1,250 2,500 5,000 O.D. 0.013 0.119 0.195 0.356 0.623 1.012 1.389 1.802 O.D. 0.013 0.119 0.195 0.356 0.623 1.012 1.389 1.802
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
