Human CCL23/MPIF-1 PicoKine® Quick ELISA Kit

About this product

Human CCL23/MPIF-1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CCL23/MPIF-1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 2 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1224

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Chemokine (C-C motif) ligand 23 (CCL23), also known as MIP-3 and MPIF-1, is a small cytokine belonging to the CC chemokine family. It is mapped to 17q12. CCL23 is predominantly expressed in lung and liver tissue, but is also found in placenta, bone marrow and some cell lines of myeloid origin. MPIF1 has chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils. It has also been attributed to an inhibitory activity on hematopoietic progenitor cells that give rise to granulocyte and monocyte lineages. Moreover, CCL23 is a ligand for the chemokine receptor CCR1.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 87 79 66 80 1.92 2.4% Sample 2 241 265 247 277 258 7.47 2.9% Sample 3 841 899 854 836 858 28.3 3.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 87 79 66 80 1.92 2.4% Sample 1 88 87 79 66 80 1.92 2.4% Sample 2 241 265 247 277 258 7.47 2.9% Sample 2 241 265 247 277 258 7.47 2.9% Sample 3 841 899 854 836 858 28.3 3.3% Sample 3 841 899 854 836 858 28.3 3.3%

Gene Name

CCL23

Gene ID

6368

UniProt

P55773

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: R22-N120

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 241 841 86 246 848 Standard deviation 6.07 18.56 74.85 5.5 21.65 70.38 CV (%) 6.9% 7.7% 8.9% 6.4% 8.8% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 241 841 86 246 848 Standard deviation 6.07 18.56 74.85 5.5 21.65 70.38 CV (%) 6.9% 7.7% 8.9% 6.4% 8.8% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 88 241 841 86 246 848 Mean (pg/ml) 88 241 841 86 246 848 Standard deviation 6.07 18.56 74.85 5.5 21.65 70.38 Standard deviation 6.07 18.56 74.85 5.5 21.65 70.38 CV (%) 6.9% 7.7% 8.9% 6.4% 8.8% 8.3% CV (%) 6.9% 7.7% 8.9% 6.4% 8.8% 8.3%

Sensitivity

<2 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Human CCL23

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.038 0.065 0.136 0.228 0.404 0.684 1.247 2.135 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.038 0.065 0.136 0.228 0.404 0.684 1.247 2.135 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.038 0.065 0.136 0.228 0.404 0.684 1.247 2.135 O.D. 0.038 0.065 0.136 0.228 0.404 0.684 1.247 2.135

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.