Mouse Fc Gamma RII/RIII (CD32/CD16) (Fcgr2b/Fcgr3) PicoKine® Quick ELISA Kit

About this product

Mouse Fc Gamma RII/RIII (CD32/CD16) (Fcgr2b/Fcgr3) PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Fc Gamma RII/RIII (CD32/CD16) (Fcgr2b/Fcgr3) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

FEK1313

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

Reproducibility

Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 87 94 85 89 4.69 5.3% Sample 2 208 235 216 244 226 11.06 4.9% Sample 3 904 867 905 841 879 39.57 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 87 94 85 89 4.69 5.3% Sample 1 88 87 94 85 89 4.69 5.3% Sample 2 208 235 216 244 226 11.06 4.9% Sample 2 208 235 216 244 226 11.06 4.9% Sample 3 904 867 905 841 879 39.57 4.5% Sample 3 904 867 905 841 879 39.57 4.5%

Gene Name

Fcgr2b/Fcgr3

Gene ID

14130/14131

UniProt

P08101/P08508

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: T40-R217

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 208 904 96 234 841 Standard deviation 3.96 12.9 29.83 5.38 14.27 58.03 CV (%) 4.5% 6.2% 3.3% 5.6% 6.1% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 208 904 96 234 841 Standard deviation 3.96 12.9 29.83 5.38 14.27 58.03 CV (%) 4.5% 6.2% 3.3% 5.6% 6.1% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 88 208 904 96 234 841 Mean (pg/ml) 88 208 904 96 234 841 Standard deviation 3.96 12.9 29.83 5.38 14.27 58.03 Standard deviation 3.96 12.9 29.83 5.38 14.27 58.03 CV (%) 4.5% 6.2% 3.3% 5.6% 6.1% 6.9% CV (%) 4.5% 6.2% 3.3% 5.6% 6.1% 6.9%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Specificity

Natural and recombinant Mouse Fcgr2b/Fcgr3

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.073 0.167 0.225 0.369 0.639 1.107 1.793 2.400 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.073 0.167 0.225 0.369 0.639 1.107 1.793 2.400 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.073 0.167 0.225 0.369 0.639 1.107 1.793 2.400 O.D. 0.073 0.167 0.225 0.369 0.639 1.107 1.793 2.400

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.