- Catalog number
- FEK1120
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chemokine (C-C motif) ligand 15 (CCL15) is a small cytokine belonging to the CC chemokine family that is also known as leukotactin-1, MIP5 and HCC-2. CCL15 is expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3. The human CCL15 gene spans four exons and is located in a head-to-tail orientation on chromosome 17 with the gene of another CC chemokine known as CCL14.
- Reproducibility
- Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 34 34 38 36 2.26 6.3% Sample 2 190 190 186 189 188 3.19 1.7% Sample 3 444 435 482 452 453 23.55 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 34 34 38 36 2.26 6.3% Sample 1 38 34 34 38 36 2.26 6.3% Sample 2 190 190 186 189 188 3.19 1.7% Sample 2 190 190 186 189 188 3.19 1.7% Sample 3 444 435 482 452 453 23.55 5.2% Sample 3 444 435 482 452 453 23.55 5.2%
- Gene Name
- CCL15
- Gene ID
- 6359
- UniProt
- Q16663
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: Q22-I113
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 190 444 36 192 422 Standard deviation 2.69 10.26 33.74 3.09 16.12 38.40 CV (%) 7.1% 5.4% 7.6% 8.6% 8.4% 9.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 190 444 36 192 422 Standard deviation 2.69 10.26 33.74 3.09 16.12 38.40 CV (%) 7.1% 5.4% 7.6% 8.6% 8.4% 9.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 38 190 444 36 192 422 Mean (pg/ml) 38 190 444 36 192 422 Standard deviation 2.69 10.26 33.74 3.09 16.12 38.40 Standard deviation 2.69 10.26 33.74 3.09 16.12 38.40 CV (%) 7.1% 5.4% 7.6% 8.6% 8.4% 9.1% CV (%) 7.1% 5.4% 7.6% 8.6% 8.4% 9.1%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece
- Storage Conditions
- Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
- Specificity
- Natural and recombinant human CCL15
- Anticoagulant
- Heparin or EDTA
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.026 0.101 0.165 0.287 0.524 0.951 1.569 2.145 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.026 0.101 0.165 0.287 0.524 0.951 1.569 2.145 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.026 0.101 0.165 0.287 0.524 0.951 1.569 2.145 O.D. 0.026 0.101 0.165 0.287 0.524 0.951 1.569 2.145
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
