- Catalog number
- EK0304
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Amphiregulin, also known as AREG, is a protein that in humans is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to 4q13.1. It is an autocrine growth factor as well as a mitogen forastrocytes, Schwann cells, fibroblasts. Amphiregulin is related to toepidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha) . This protein interacts with the Epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis-like skin phenotype, and its expression was increased in parallel with typical Th2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty-induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 29 27 27 27 0.82 3% Sample 2 200 207 196 193 199 5.24 2.6% Sample 3 441 428 410 450 432 15.03 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 29 27 27 27 0.82 3% Sample 1 28 29 27 27 27 0.82 3% Sample 2 200 207 196 193 199 5.24 2.6% Sample 2 200 207 196 193 199 5.24 2.6% Sample 3 441 428 410 450 432 15.03 3.4% Sample 3 441 428 410 450 432 15.03 3.4%
- Synonyms
- Amphiregulin; AR; Colorectum cell-derived growth factor; CRDGF; AREG; SDGF; AREGB
- Gene Name
- AREG
- Gene ID
- 374
- UniProt
- P15514
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: S101-K198
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Growth Factors, Growth Factors/Hormones, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and human milk.
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 200 441 30 197 433 Standard deviation 1.65 10.4 29.54 2.19 10.63 31.6 CV (%) 6.7%% 5.2% 6.7% 7.3% 5.4% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 200 441 30 197 433 Standard deviation 1.65 10.4 29.54 2.19 10.63 31.6 CV (%) 6.7%% 5.2% 6.7% 7.3% 5.4% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 28 200 441 30 197 433 Mean (pg/ml) 28 200 441 30 197 433 Standard deviation 1.65 10.4 29.54 2.19 10.63 31.6 Standard deviation 1.65 10.4 29.54 2.19 10.63 31.6 CV (%) 6.7%% 5.2% 6.7% 7.3% 5.4% 7.3% CV (%) 6.7%% 5.2% 6.7% 7.3% 5.4% 7.3%
- Sensitivity
- <10 pg/mL
- Function
- Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 27895 MW
- Specificity
- Natural and recombinant human Amphiregulin
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Amphiregulin
- Subcellular Location
- Membrane; Single-pass membrane protein.
- Sequence Similarities
- Belongs to the amphiregulin family.
- Protein Name
- Amphiregulin
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.018 0.039 0.063 0.113 0.219 0.451 0.979 1.863 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.018 0.039 0.063 0.113 0.219 0.451 0.979 1.863 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.018 0.039 0.063 0.113 0.219 0.451 0.979 1.863 O.D. 0.018 0.039 0.063 0.113 0.219 0.451 0.979 1.863
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
