- Catalog number
- EK0307
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF) . BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP) . BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 47 56 51 52 51 3.2 6.2% Sample 2 224 188 221 204 209 14.44 6.9% Sample 3 1022 1010 870 1050 988 69.65 7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 47 56 51 52 51 3.2 6.2% Sample 1 47 56 51 52 51 3.2 6.2% Sample 2 224 188 221 204 209 14.44 6.9% Sample 2 224 188 221 204 209 14.44 6.9% Sample 3 1022 1010 870 1050 988 69.65 7% Sample 3 1022 1010 870 1050 988 69.65 7%
- Synonyms
- Brain-derived neurotrophic factor; BDNF; Abrineurin; BDNF
- Gene Name
- BDNF
- Gene ID
- 627
- UniProt
- P23560
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: H129-R247
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated monoclonal antibody from mouse
- Tissue Specificity
- Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. .
- Applications
- ELISA
- Field of Research
- Alzheimer's Disease, Atherosclerosis, Cardiovascular, Diabetes, Diabetes-associated, Growth and Development, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Obesity
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 47 224 1022 44 244 926 Standard deviation 3.57 9.63 44.96 3.78 12.44 50 CV (%) 7.6% 4.3% 4.4% 8.6% 5.1% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 47 224 1022 44 244 926 Standard deviation 3.57 9.63 44.96 3.78 12.44 50 CV (%) 7.6% 4.3% 4.4% 8.6% 5.1% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 47 224 1022 44 244 926 Mean (pg/ml) 47 224 1022 44 244 926 Standard deviation 3.57 9.63 44.96 3.78 12.44 50 Standard deviation 3.57 9.63 44.96 3.78 12.44 50 CV (%) 7.6% 4.3% 4.4% 8.6% 5.1% 5.4% CV (%) 7.6% 4.3% 4.4% 8.6% 5.1% 5.4%
- Sensitivity
- <15 pg/mL
- Function
- During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 27818 MW
- Specificity
- Natural and recombinant human BDNF
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Brain-derived neurotrophic factor
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the NGF-beta family.
- Protein Name
- Brain-derived neurotrophic factor
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.022 0.074 0.115 0.222 0.423 0.707 1.205 1.93 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.022 0.074 0.115 0.222 0.423 0.707 1.205 1.93 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.022 0.074 0.115 0.222 0.423 0.707 1.205 1.93 O.D. 0.022 0.074 0.115 0.222 0.423 0.707 1.205 1.93
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
