- Catalog number
- EK0331
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chemokine (C-C motif) ligand 24 (CCL24) also known as myeloid progenitor inhibitory factor 2 (MPIF-2) or eosinophil chemotactic protein 2 (eotaxin-2) is a protein that in humans is encoded by the CCL24 gene. By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23. CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 82 86 80 80 3.96 4.9% Sample 2 209 190 207 207 203 7.69 3.7% Sample 3 857 1006 836 933 908 67.1 7.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 82 86 80 80 3.96 4.9% Sample 1 75 82 86 80 80 3.96 4.9% Sample 2 209 190 207 207 203 7.69 3.7% Sample 2 209 190 207 207 203 7.69 3.7% Sample 3 857 1006 836 933 908 67.1 7.3% Sample 3 857 1006 836 933 908 67.1 7.3%
- Synonyms
- C-C motif chemokine 24; CK-beta-6; Eosinophil chemotactic protein 2; Eotaxin-2; Myeloid progenitor inhibitory factor 2; MPIF-2; Small-inducible cytokine A24; CCL24; MPIF2, SCYA24
- Gene Name
- CCL24
- Gene ID
- 6369
- UniProt
- O00175
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: V27-C119
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Activated monocytes and activated T lymphocytes.
- Applications
- ELISA
- Field of Research
- Chemokines, Immunology, Innate Immunity
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 75 209 857 70 228 777 Standard deviation 3.67 13.16 44.56 4.13 16.41 41.18 CV (%) 4.9% 6.3% 5.2% 5.9% 7.2% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 75 209 857 70 228 777 Standard deviation 3.67 13.16 44.56 4.13 16.41 41.18 CV (%) 4.9% 6.3% 5.2% 5.9% 7.2% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 75 209 857 70 228 777 Mean (pg/ml) 75 209 857 70 228 777 Standard deviation 3.67 13.16 44.56 4.13 16.41 41.18 Standard deviation 3.67 13.16 44.56 4.13 16.41 41.18 CV (%) 4.9% 6.3% 5.2% 5.9% 7.2% 5.3% CV (%) 4.9% 6.3% 5.2% 5.9% 7.2% 5.3%
- Sensitivity
- <2 pg/mL
- Function
- Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 13134 MW
- Specificity
- Natural and recombinant human CCL24
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-C motif chemokine 24
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the intercrine beta (chemokine CC) family.
- Protein Name
- C-C motif chemokine 24
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.018 0.054 0.095 0.189 0.358 0.759 1.468 2.797 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.018 0.054 0.095 0.189 0.358 0.759 1.468 2.797 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.018 0.054 0.095 0.189 0.358 0.759 1.468 2.797 O.D. 0.018 0.054 0.095 0.189 0.358 0.759 1.468 2.797
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
