- Catalog number
- EK0333
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. Its gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What's more, EPO is also involved in the wound healing process.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 111 109 96 102 104 5.93 5.7% Sample 2 307 322 308 317 313 6.26 2% Sample 3 1298 1321 1280 1385 1321 39.7 3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 111 109 96 102 104 5.93 5.7% Sample 1 111 109 96 102 104 5.93 5.7% Sample 2 307 322 308 317 313 6.26 2% Sample 2 307 322 308 317 313 6.26 2% Sample 3 1298 1321 1280 1385 1321 39.7 3% Sample 3 1298 1321 1280 1385 1321 39.7 3%
- Synonyms
- Erythropoietin; Epo
- Gene Name
- EPO
- Gene ID
- 13856
- UniProt
- P07321
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A27-R192
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Monoclonal antibody from rat
- Tissue Specificity
- Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
- Applications
- ELISA
- Field of Research
- Angiogenesis, Apoptosis, Atherosclerosis, Cardiovascular, G Protein Signaling, Hematopoietic Progenitors, Hypertrophy, Immunoglobulins, Immunology, Receptors, Signal Transduction, Signaling Pathway, Small G Proteins, Stem Cells, Surface Molecules
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin) .
- Detection Range
- 46.9 pg/mL - 3,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 111 307 1298 106 278 1216 Standard deviation 7.87 17.19 55.81 7.86 16.68 71.74 CV (%) 7.1% 5.6% 4.3% 7.8% 6% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 111 307 1298 106 278 1216 Standard deviation 7.87 17.19 55.81 7.86 16.68 71.74 CV (%) 7.1% 5.6% 4.3% 7.8% 6% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 111 307 1298 106 278 1216 Mean (pg/ml) 111 307 1298 106 278 1216 Standard deviation 7.87 17.19 55.81 7.86 16.68 71.74 Standard deviation 7.87 17.19 55.81 7.86 16.68 71.74 CV (%) 7.1% 5.6% 4.3% 7.8% 6% 5.9% CV (%) 7.1% 5.6% 4.3% 7.8% 6% 5.9%
- Sensitivity
- <15 pg/mL
- Function
- Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 21365 MW
- Specificity
- Natural and recombinant mouse EPO
- Anticoagulant
- Heparin
- Gene Name Synonym
- Erythropoietin
- Subcellular Location
- Secreted.
- Protein Name
- Erythropoietin
- Sample Data
- Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.023 0.073 0.102 0.185 0.357 0.612 1.138 1.901 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.023 0.073 0.102 0.185 0.357 0.612 1.138 1.901 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.023 0.073 0.102 0.185 0.357 0.612 1.138 1.901 O.D. 0.023 0.073 0.102 0.185 0.357 0.612 1.138 1.901
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
