- Catalog number
- EK0336
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor (NGF) /tumour necrosis factor (TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48-kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 67 72 66 74 69 3.34 4.8% Sample 2 227 219 205 237 222 11.7 5.2% Sample 3 869 913 935 934 912 26.74 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 67 72 66 74 69 3.34 4.8% Sample 1 67 72 66 74 69 3.34 4.8% Sample 2 227 219 205 237 222 11.7 5.2% Sample 2 227 219 205 237 222 11.7 5.2% Sample 3 869 913 935 934 912 26.74 2.9% Sample 3 869 913 935 934 912 26.74 2.9%
- Synonyms
- Tumor necrosis factor receptor superfamily member 6 ; Fas
- Gene Name
- FAS
- Gene ID
- 14102
- UniProt
- Q8C350
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q22-R169
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Apoptosis, Autoimmune, Cell Biology, Cytokines, Hematopoietic Progenitors, Immune System Diseases, Immunology, Innate Immunity, Lymphoid, Stem Cells, T Lymphocytic Lineage, TNF Superfamily
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 67 227 869 60 222 905 Standard deviation 3.35 14.07 63.43 3.66 17.76 70.59 CV (%) 5% 6.2% 7.3% 6.1% 8% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 67 227 869 60 222 905 Standard deviation 3.35 14.07 63.43 3.66 17.76 70.59 CV (%) 5% 6.2% 7.3% 6.1% 8% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 67 227 869 60 222 905 Mean (pg/ml) 67 227 869 60 222 905 Standard deviation 3.35 14.07 63.43 3.66 17.76 70.59 Standard deviation 3.35 14.07 63.43 3.66 17.76 70.59 CV (%) 5% 6.2% 7.3% 6.1% 8% 7.8% CV (%) 5% 6.2% 7.3% 6.1% 8% 7.8%
- Sensitivity
- <3 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 7116 MW
- Specificity
- Natural and recombinant mouse FAS
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Fas cell surface death receptor
- Protein Name
- Tumor necrosis factor receptor superfamily member 6
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.049 0.182 0.253 0.375 0.539 0.855 1.355 1.997 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.049 0.182 0.253 0.375 0.539 0.855 1.355 1.997 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.049 0.182 0.253 0.375 0.539 0.855 1.355 1.997 O.D. 0.049 0.182 0.253 0.375 0.539 0.855 1.355 1.997
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
