- Catalog number
- EK0337
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Fas ligand (FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the target cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 41 41 34 38 3.08 8.1% Sample 2 186 197 192 198 193 4.76 2.4% Sample 3 437 495 446 507 471 30.21 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 41 41 34 38 3.08 8.1% Sample 1 36 41 41 34 38 3.08 8.1% Sample 2 186 197 192 198 193 4.76 2.4% Sample 2 186 197 192 198 193 4.76 2.4% Sample 3 437 495 446 507 471 30.21 6.4% Sample 3 437 495 446 507 471 30.21 6.4%
- Synonyms
- Tumor necrosis factor ligand superfamily member 6; Apoptosis antigen ligand; APTL; CD95 ligand; CD95-L; Fas antigen ligand; Fas ligand; FasL; CD178; Tumor necrosis factor ligand superfamily member 6, membrane form; Tumor necrosis factor ligand superfamily member 6, soluble form; Receptor-binding FasL ectodomain; Soluble Fas ligand; sFasL; ADAM10-processed FasL form; APL; FasL intracellular domain; FasL ICD; SPPL2A-processed FasL form; SPA; FASLG; APT1LG1, CD95L, FASL, TNFSF6
- Gene Name
- FASLG
- Gene ID
- 356
- UniProt
- P48023
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: P134-L281
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Apoptosis, Caspases, Cell Biology, Cytokines, Hematopoietic Progenitors, Immunology, Innate Immunity, Intracellular, Lymphoid, Receptors, Stem Cells, T Lymphocytic Lineage, TNF Superfamily
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 186 437 34 189 419 Standard deviation 2.19 9.67 28.84 2.1 12.47 33.1 CV (%) 6.1% 5.2% 6.6% 6.2% 6.6% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 186 437 34 189 419 Standard deviation 2.19 9.67 28.84 2.1 12.47 33.1 CV (%) 6.1% 5.2% 6.6% 6.2% 6.6% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 186 437 34 189 419 Mean (pg/ml) 36 186 437 34 189 419 Standard deviation 2.19 9.67 28.84 2.1 12.47 33.1 Standard deviation 2.19 9.67 28.84 2.1 12.47 33.1 CV (%) 6.1% 5.2% 6.6% 6.2% 6.6% 7.9% CV (%) 6.1% 5.2% 6.6% 6.2% 6.6% 7.9%
- Sensitivity
- <2 pg/mL
- Function
- Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:26334989, PubMed:9228058) . Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed:9228058, PubMed:7528780, PubMed:9427603) . Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity) . TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity) . Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed:27806260) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 31485 MW
- Specificity
- Natural and recombinant human FASL
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Tumor necrosis factor ligand superfamily member 6
- Subcellular Location
- Cell membrane ; Single-pass type II membrane protein . Cytoplasmic vesicle lumen . Lysosome lumen . Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination (PubMed:17164290) . Colocalizes with the SPPL2A protease at the cell membrane (PubMed:17557115) . .
- Protein Name
- Tumor necrosis factor ligand superfamily member 6
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.084 0.142 0.267 0.527 0.912 1.465 2.273 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.084 0.142 0.267 0.527 0.912 1.465 2.273 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.084 0.142 0.267 0.527 0.912 1.465 2.273 O.D. 0.023 0.084 0.142 0.267 0.527 0.912 1.465 2.273
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
