- Catalog number
- EK0354
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Fms-related tyrosine kinase 3 ligand (FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1) . In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells (DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 37 39 40 38 1.29 3.3% Sample 2 186 184 208 185 190 9.98 5.2% Sample 3 439 486 448 470 460 18.43 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 37 39 40 38 1.29 3.3% Sample 1 37 37 39 40 38 1.29 3.3% Sample 2 186 184 208 185 190 9.98 5.2% Sample 2 186 184 208 185 190 9.98 5.2% Sample 3 439 486 448 470 460 18.43 4% Sample 3 439 486 448 470 460 18.43 4%
- Synonyms
- Fms-related tyrosine kinase 3 ligand; Flt3 ligand; Flt3L; SL cytokine; FLT3LG
- Gene Name
- FLT3LG
- Gene ID
- 2323
- UniProt
- P49771
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: T27-P185
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cardiovascular, Cytokines, Immunology, Innate Immunity
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 186 439 38 191 431 Standard deviation 2.81 11.16 34.24 3.45 13.75 38.35 CV (%) 7.6% 6% 7.8% 9.1% 7.2% 8.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 186 439 38 191 431 Standard deviation 2.81 11.16 34.24 3.45 13.75 38.35 CV (%) 7.6% 6% 7.8% 9.1% 7.2% 8.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 37 186 439 38 191 431 Mean (pg/ml) 37 186 439 38 191 431 Standard deviation 2.81 11.16 34.24 3.45 13.75 38.35 Standard deviation 2.81 11.16 34.24 3.45 13.75 38.35 CV (%) 7.6% 6% 7.8% 9.1% 7.2% 8.9% CV (%) 7.6% 6% 7.8% 9.1% 7.2% 8.9%
- Sensitivity
- <10 pg/mL
- Function
- Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 26416 MW
- Specificity
- Natural and recombinant human Flt-3ligand
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Fms-related tyrosine kinase 3 ligand
- Subcellular Location
- Isoform 1: Cell membrane; Single-pass type I membrane protein.
- Protein Name
- Fms-related tyrosine kinase 3 ligand
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.041 0.111 0.187 0.367 0.672 1.066 1.665 2.033 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.041 0.111 0.187 0.367 0.672 1.066 1.665 2.033 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.041 0.111 0.187 0.367 0.672 1.066 1.665 2.033 O.D. 0.041 0.111 0.187 0.367 0.672 1.066 1.665 2.033
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
