- Catalog number
- EK0360
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17. The standard product used in this kit is recombinant human G-CSF, consisting of 175 amino acids with the molecular mass of 18.8KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 62 57 60 56 58 2.38 4.1% Sample 2 237 209 236 225 226 11.27 4.9% Sample 3 1001 964 888 983 959 43.02 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 62 57 60 56 58 2.38 4.1% Sample 1 62 57 60 56 58 2.38 4.1% Sample 2 237 209 236 225 226 11.27 4.9% Sample 2 237 209 236 225 226 11.27 4.9% Sample 3 1001 964 888 983 959 43.02 4.4% Sample 3 1001 964 888 983 959 43.02 4.4%
- Synonyms
- Granulocyte colony-stimulating factor; G-CSF; Pluripoietin; Filgrastim; Lenograstim; CSF3; C17orf33, GCSF
- Gene Name
- CSF3
- Gene ID
- 1440
- UniProt
- P09919
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: T31-P204
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cytokines, Immunology, Innate Immunity, Invasion/Microenvironment
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 62 237 1001 64 230 966 Standard deviation 3.96 16.26 45.04 4.99 18.17 59.89 CV (%) 6.4% 6.4% 4.5% 7.8% 7.9% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 62 237 1001 64 230 966 Standard deviation 3.96 16.26 45.04 4.99 18.17 59.89 CV (%) 6.4% 6.4% 4.5% 7.8% 7.9% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 62 237 1001 64 230 966 Mean (pg/ml) 62 237 1001 64 230 966 Standard deviation 3.96 16.26 45.04 4.99 18.17 59.89 Standard deviation 3.96 16.26 45.04 4.99 18.17 59.89 CV (%) 6.4% 6.4% 4.5% 7.8% 7.9% 6.2% CV (%) 6.4% 6.4% 4.5% 7.8% 7.9% 6.2%
- Sensitivity
- <4 pg/mL
- Function
- Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 22293 MW
- Specificity
- Natural and recombinant human G-CSF
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Granulocyte colony-stimulating factor
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the IL-6 superfamily.
- Protein Name
- Granulocyte colony-stimulating factor
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.088 0.15 0.199 0.307 0.484 0.829 1.454 2.558 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.088 0.15 0.199 0.307 0.484 0.829 1.454 2.558 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.088 0.15 0.199 0.307 0.484 0.829 1.454 2.558 O.D. 0.088 0.15 0.199 0.307 0.484 0.829 1.454 2.558
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
