- Catalog number
- EK0364
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts ly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 30 31 32 30 1.47 4.9% Sample 2 213 209 210 221 213 4.71 2.2% Sample 3 388 374 388 433 395 22.25 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 30 31 32 30 1.47 4.9% Sample 1 28 30 31 32 30 1.47 4.9% Sample 2 213 209 210 221 213 4.71 2.2% Sample 2 213 209 210 221 213 4.71 2.2% Sample 3 388 374 388 433 395 22.25 5.6% Sample 3 388 374 388 433 395 22.25 5.6%
- Synonyms
- Granulocyte-macrophage colony-stimulating factor; GM-CSF; Colony-stimulating factor; CSF; Molgramostin; Sargramostim; CSF2; GMCSF
- Gene Name
- CSF2
- Gene ID
- 1437
- UniProt
- P04141
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A18-E144
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cytokines, Immunology, Innate Immunity
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 213 388 27 219 407 Standard deviation 1.34 15.76 19.78 1.8 20.14 26.04 CV (%) 4.8% 7.4% 6.4% 6.7% 9.2% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 213 388 27 219 407 Standard deviation 1.34 15.76 19.78 1.8 20.14 26.04 CV (%) 4.8% 7.4% 6.4% 6.7% 9.2% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 28 213 388 27 219 407 Mean (pg/ml) 28 213 388 27 219 407 Standard deviation 1.34 15.76 19.78 1.8 20.14 26.04 Standard deviation 1.34 15.76 19.78 1.8 20.14 26.04 CV (%) 4.8% 7.4% 6.4% 6.7% 9.2% 6.4% CV (%) 4.8% 7.4% 6.4% 6.7% 9.2% 6.4%
- Sensitivity
- <1 pg/mL
- Function
- Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 16295 MW
- Specificity
- Natural and recombinant human GM-CSF
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Granulocyte-macrophage colony-stimulating factor
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the GM-CSF family.
- Protein Name
- Granulocyte-macrophage colony-stimulating factor
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.024 0.086 0.154 0.292 0.538 0.988 1.598 2.115 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.024 0.086 0.154 0.292 0.538 0.988 1.598 2.115 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.024 0.086 0.154 0.292 0.538 0.988 1.598 2.115 O.D. 0.024 0.086 0.154 0.292 0.538 0.988 1.598 2.115
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
