- Catalog number
- EK0366
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts ly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 33 34 29 31 2.06 6.6% Sample 2 216 227 243 239 231 10.59 4.5% Sample 3 429 381 390 404 401 18.12 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 33 34 29 31 2.06 6.6% Sample 1 30 33 34 29 31 2.06 6.6% Sample 2 216 227 243 239 231 10.59 4.5% Sample 2 216 227 243 239 231 10.59 4.5% Sample 3 429 381 390 404 401 18.12 4.5% Sample 3 429 381 390 404 401 18.12 4.5%
- Synonyms
- Granulocyte-macrophage colony-stimulating factor; GM-CSF; Colony-stimulating factor; CSF; Csf2; Csfgm
- Gene Name
- CSF2
- Gene ID
- 116630
- UniProt
- P48750
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A1-K127
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cytokines, Immunology, Innate Immunity
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 216 429 28 196 414 Standard deviation 1.35 11.44 33.89 1.59 12.34 39.33 CV (%) 4.5% 5.3% 7.9% 5.7% 6.3% 9.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 216 429 28 196 414 Standard deviation 1.35 11.44 33.89 1.59 12.34 39.33 CV (%) 4.5% 5.3% 7.9% 5.7% 6.3% 9.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 30 216 429 28 196 414 Mean (pg/ml) 30 216 429 28 196 414 Standard deviation 1.35 11.44 33.89 1.59 12.34 39.33 Standard deviation 1.35 11.44 33.89 1.59 12.34 39.33 CV (%) 4.5% 5.3% 7.9% 5.7% 6.3% 9.5% CV (%) 4.5% 5.3% 7.9% 5.7% 6.3% 9.5%
- Sensitivity
- <10 pg/mL
- Function
- Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 16562 MW
- Specificity
- Natural and recombinant rat GM-CSF
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Granulocyte-macrophage colony-stimulating factor
- Subcellular Location
- Secreted.
- Protein Name
- Granulocyte-macrophage colony-stimulating factor
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.044 0.095 0.135 0.212 0.351 0.6253 1.155 1.885 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.044 0.095 0.135 0.212 0.351 0.6253 1.155 1.885 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.044 0.095 0.135 0.212 0.351 0.6253 1.155 1.885 O.D. 0.044 0.095 0.135 0.212 0.351 0.6253 1.155 1.885
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
