- Catalog number
- EK0367
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- GP130 (Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein?which is the founding member of the class of all?cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130?is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membrane-bound?gp130. Gp130 is an important part of many different types of signaling complexes. Gp130 molecules with mutations in either of these segments could not transduce a growth signal.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 186 203 198 207 198 7.88 3.9% Sample 2 1115 1150 1073 1056 1098 36.67 3.3% Sample 3 3384 3507 3537 3330 3439 85.34 2.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 186 203 198 207 198 7.88 3.9% Sample 1 186 203 198 207 198 7.88 3.9% Sample 2 1115 1150 1073 1056 1098 36.67 3.3% Sample 2 1115 1150 1073 1056 1098 36.67 3.3% Sample 3 3384 3507 3537 3330 3439 85.34 2.4% Sample 3 3384 3507 3537 3330 3439 85.34 2.4%
- Synonyms
- Interleukin-6 receptor subunit beta; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; CDw130; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; Oncostatin-M receptor subunit alpha; CD130; IL6ST
- Gene Name
- IL6ST
- Gene ID
- 3572
- UniProt
- P40189
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L24-E619
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
- Applications
- ELISA
- Field of Research
- Adapters, Apoptosis, Associated Proteins, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cell Type Markers, Cytokines, Hypertrophy, Immunology, Intracellular, Signal Transduction, Transmembrane
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 125 pg/mL - 8,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 186 1115 3384 178 1076 3150 Standard deviation 13.2 73.59 156.6%6 12.63 80.7 198.45 CV (%) 7.1% 6.6% 4.6% 7.1% 7.5% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 186 1115 3384 178 1076 3150 Standard deviation 13.2 73.59 156.6%6 12.63 80.7 198.45 CV (%) 7.1% 6.6% 4.6% 7.1% 7.5% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 186 1115 3384 178 1076 3150 Mean (pg/ml) 186 1115 3384 178 1076 3150 Standard deviation 13.2 73.59 156.6%6 12.63 80.7 198.45 Standard deviation 13.2 73.59 156.6%6 12.63 80.7 198.45 CV (%) 7.1% 6.6% 4.6% 7.1% 7.5% 6.3% CV (%) 7.1% 6.6% 4.6% 7.1% 7.5% 6.3%
- Sensitivity
- <10 pg/mL
- Function
- Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity) . The type I OSM receptor is capable of transducing OSM-specific signaling events. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 103537 MW
- Specificity
- Natural and recombinant human Sgp130
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Interleukin-6 receptor subunit beta
- Subcellular Location
- Isoform 1: Cell membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Belongs to the type I cytokine receptor family. Type 2 subfamily.
- Protein Name
- Interleukin-6 receptor subunit beta
- Sample Data
- Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.029 0.078 0.143 0.234 0.424 0.846 1.289 1.909 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.029 0.078 0.143 0.234 0.424 0.846 1.289 1.909 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.029 0.078 0.143 0.234 0.424 0.846 1.289 1.909 O.D. 0.029 0.078 0.143 0.234 0.424 0.846 1.289 1.909
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
