- Catalog number
- EK0370
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1) . ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 260 271 261 266 5.52 2% Sample 2 1923 1695 1731 1759 1777 87.29 4.9% Sample 3 4746 4742 4337 4571 4599 166.94 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 260 271 261 266 5.52 2% Sample 1 272 260 271 261 266 5.52 2% Sample 2 1923 1695 1731 1759 1777 87.29 4.9% Sample 2 1923 1695 1731 1759 1777 87.29 4.9% Sample 3 4746 4742 4337 4571 4599 166.94 3.6% Sample 3 4746 4742 4337 4571 4599 166.94 3.6%
- Synonyms
- Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD54; ICAM1
- Gene Name
- ICAM1
- Gene ID
- 3383
- UniProt
- P05362
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q28-E480
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Atherosclerosis, Cardiovascular, Cell Adhesion Molecules, Cell Type Markers, Hematopoietic Progenitors, Immunology, Lymphoid, Stem Cells, T Lymphocytic Lineage, Vascular Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1923 4746 248 1929 4549 Standard deviation 14.41 111.53 204.07 14.13 125.38 236.54 CV (%) 5.3% 5.8% 4.3% 5.7% 6.5% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1923 4746 248 1929 4549 Standard deviation 14.41 111.53 204.07 14.13 125.38 236.54 CV (%) 5.3% 5.8% 4.3% 5.7% 6.5% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 272 1923 4746 248 1929 4549 Mean (pg/ml) 272 1923 4746 248 1929 4549 Standard deviation 14.41 111.53 204.07 14.13 125.38 236.54 Standard deviation 14.41 111.53 204.07 14.13 125.38 236.54 CV (%) 5.3% 5.8% 4.3% 5.7% 6.5% 5.2% CV (%) 5.3% 5.8% 4.3% 5.7% 6.5% 5.2%
- Sensitivity
- <10 pg/mL
- Function
- ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) . During leukocyte trans- endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 57825 MW
- Specificity
- Natural and recombinant human sICAM-1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Intercellular adhesion molecule 1
- Subcellular Location
- Membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Belongs to the immunoglobulin superfamily. ICAM family.
- Protein Name
- Intercellular adhesion molecule 1
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.097 0.151 0.242 0.434 0.843 1.489 2.184 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.097 0.151 0.242 0.434 0.843 1.489 2.184 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.097 0.151 0.242 0.434 0.843 1.489 2.184 O.D. 0.056 0.097 0.151 0.242 0.434 0.843 1.489 2.184
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
