- Catalog number
- EK0385
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Insulin-like growth factor-binding protein 2 (IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 160 171 174 173 169 5.59 3.3% Sample 2 970 1024 949 988 982 27.52 2.8% Sample 3 3281 3102 2889 3320 3148 170.63 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 160 171 174 173 169 5.59 3.3% Sample 1 160 171 174 173 169 5.59 3.3% Sample 2 970 1024 949 988 982 27.52 2.8% Sample 2 970 1024 949 988 982 27.52 2.8% Sample 3 3281 3102 2889 3320 3148 170.63 5.4% Sample 3 3281 3102 2889 3320 3148 170.63 5.4%
- Synonyms
- Insulin-like growth factor-binding protein 2; IBP-2; IGF-binding protein 2; IGFBP-2; mIGFBP-2; Igfbp2; Igfbp-2
- Gene Name
- IGFBP2
- Gene ID
- 16008
- UniProt
- P47877
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E35-Q305
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Highly expressed in adult liver, but also in kidney, lung, brain, spleen, testis and ovary. .
- Applications
- ELISA
- Field of Research
- Cancer, Extracellular Matrix, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 125 pg/mL - 8,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 160 970 3281 169 968 3467 Standard deviation 7.52 76.63 203.42 8.95 86.15 256.55 CV (%) 4.7% 7.9% 6.2% 5.3% 8.9% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 160 970 3281 169 968 3467 Standard deviation 7.52 76.63 203.42 8.95 86.15 256.55 CV (%) 4.7% 7.9% 6.2% 5.3% 8.9% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 160 970 3281 169 968 3467 Mean (pg/ml) 160 970 3281 169 968 3467 Standard deviation 7.52 76.63 203.42 8.95 86.15 256.55 Standard deviation 7.52 76.63 203.42 8.95 86.15 256.55 CV (%) 4.7% 7.9% 6.2% 5.3% 8.9% 7.4% CV (%) 4.7% 7.9% 6.2% 5.3% 8.9% 7.4%
- Sensitivity
- <10 pg/mL
- Function
- Inhibits IGF-mediated growth and developmental rates (By similarity) . IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 32847 MW
- Specificity
- Natural and recombinant mouse IGFBP2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Insulin-like growth factor-binding protein 2
- Subcellular Location
- Secreted.
- Sequence Similarities
- Contains 1 IGFBP N-terminal domain.
- Protein Name
- Insulin-like growth factor-binding protein 2
- Sample Data
- Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.083 0.135 0.182 0.243 0.43 0.773 1.277 1.958 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.083 0.135 0.182 0.243 0.43 0.773 1.277 1.958 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.083 0.135 0.182 0.243 0.43 0.773 1.277 1.958 O.D. 0.083 0.135 0.182 0.243 0.43 0.773 1.277 1.958
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
