- Catalog number
- EK0390
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin (IL) -1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant rat IL-1alpha with the molecular mass of 18kDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 15 16 15 17 15 0.82 5.4% Sample 2 40 39 46 45 42 3.04 7.2% Sample 3 131 123 146 136 134 8.33 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 15 16 15 17 15 0.82 5.4% Sample 1 15 16 15 17 15 0.82 5.4% Sample 2 40 39 46 45 42 3.04 7.2% Sample 2 40 39 46 45 42 3.04 7.2% Sample 3 131 123 146 136 134 8.33 6.2% Sample 3 131 123 146 136 134 8.33 6.2%
- Synonyms
- Interleukin-1 alpha; IL-1 alpha; Il1a
- Gene Name
- IL1A
- Gene ID
- 24493
- UniProt
- P16598
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: S115-S270
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 4.69 pg/mL - 300 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 15 40 131 14 43 120 Standard deviation 1.06 2.12 9.69 1.09 2.58 10.8 CV (%) 7.1% 5.3% 7.4% 7.8% 6% 9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 15 40 131 14 43 120 Standard deviation 1.06 2.12 9.69 1.09 2.58 10.8 CV (%) 7.1% 5.3% 7.4% 7.8% 6% 9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 15 40 131 14 43 120 Mean (pg/ml) 15 40 131 14 43 120 Standard deviation 1.06 2.12 9.69 1.09 2.58 10.8 Standard deviation 1.06 2.12 9.69 1.09 2.58 10.8 CV (%) 7.1% 5.3% 7.4% 7.8% 6% 9% CV (%) 7.1% 5.3% 7.4% 7.8% 6% 9%
- Sensitivity
- <0.5 pg/mL
- Function
- Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 30856 MW
- Specificity
- Natural and recombinant rat IL-1 alpha
- Gene Name Synonym
- Interleukin-1 alpha
- Subcellular Location
- Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
- Protein Name
- Interleukin-1 alpha
- Sample Data
- Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.008 0.1 0.172 0.327 0.665 0.986 1.556 1.844 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.008 0.1 0.172 0.327 0.665 0.986 1.556 1.844 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.008 0.1 0.172 0.327 0.665 0.986 1.556 1.844 O.D. 0.008 0.1 0.172 0.327 0.665 0.986 1.556 1.844
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
