Mouse IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine®

About this product

Mouse IL-1 Alpha ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Il1a in cell culture supernatants and serum. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0391

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin (IL-1alpha and IL-1beta) have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant mouse IL-1alpha with the molecular mass of 18kDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 15 16 16 13 15 1.22 8.1% Sample 2 63 68 60 67 64 3.2 5% Sample 3 151 139 139 146 143 5.06 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 15 16 16 13 15 1.22 8.1% Sample 1 15 16 16 13 15 1.22 8.1% Sample 2 63 68 60 67 64 3.2 5% Sample 2 63 68 60 67 64 3.2 5% Sample 3 151 139 139 146 143 5.06 3.5% Sample 3 151 139 139 146 143 5.06 3.5%

Synonyms

Interleukin-1 alpha; IL-1 alpha; Il1a

Gene Name

IL1A

Gene ID

16175

UniProt

P01582

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S115-S270

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants and serum.

Detection Range

4.7 pg/mL - 300 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 15 63 151 16 60 155 Standard deviation 1 2.7 6.64 1.18 3.3 7.28 CV (%) 6.7% 4.3% 4.4% 7.4% 5.5% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 15 63 151 16 60 155 Standard deviation 1 2.7 6.64 1.18 3.3 7.28 CV (%) 6.7% 4.3% 4.4% 7.4% 5.5% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 15 63 151 16 60 155 Mean (pg/ml) 15 63 151 16 60 155 Standard deviation 1 2.7 6.64 1.18 3.3 7.28 Standard deviation 1 2.7 6.64 1.18 3.3 7.28 CV (%) 6.7% 4.3% 4.4% 7.4% 5.5% 4.7% CV (%) 6.7% 4.3% 4.4% 7.4% 5.5% 4.7%

Sensitivity

<1 pg/mL

Function

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

31023 MW

Specificity

Natural and recombinant mouse IL-1 alpha

Gene Name Synonym

Interleukin-1 alpha

Subcellular Location

Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Protein Name

Interleukin-1 alpha

Sample Data

Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.04 0.106 0.174 0.305 0.573 1.028 1.683 2.231 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.04 0.106 0.174 0.305 0.573 1.028 1.683 2.231 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.04 0.106 0.174 0.305 0.573 1.028 1.683 2.231 O.D. 0.04 0.106 0.174 0.305 0.573 1.028 1.683 2.231

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.