- Catalog number
- EK0394
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB) -regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 31 32 32 1.11 3.4% Sample 2 211 206 203 208 207 2.91 1.4% Sample 3 483 452 433 430 449 21.1 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 31 32 32 1.11 3.4% Sample 1 33 34 31 32 32 1.11 3.4% Sample 2 211 206 203 208 207 2.91 1.4% Sample 2 211 206 203 208 207 2.91 1.4% Sample 3 483 452 433 430 449 21.1 4.6% Sample 3 483 452 433 430 449 21.1 4.6%
- Synonyms
- Interleukin-1 beta; IL-1 beta; IL1B
- Gene Name
- IL1B
- Gene ID
- 16176
- UniProt
- P10749
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: V118-S269
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Atherosclerosis, Cardiovascular, Cytokines, Immunology, Innate Immunity, Interleukins, Metabolism, Obesity, Vascular Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 12.5 pg/mL - 800 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 211 483 36 217 481 Standard deviation 1.74 10.33 34.29 2.19 14.75 35.59 CV (%) 5.3% 4.9% 7.1% 6.1% 6.8% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 211 483 36 217 481 Standard deviation 1.74 10.33 34.29 2.19 14.75 35.59 CV (%) 5.3% 4.9% 7.1% 6.1% 6.8% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 211 483 36 217 481 Mean (pg/ml) 33 211 483 36 217 481 Standard deviation 1.74 10.33 34.29 2.19 14.75 35.59 Standard deviation 1.74 10.33 34.29 2.19 14.75 35.59 CV (%) 5.3% 4.9% 7.1% 6.1% 6.8% 7.4% CV (%) 5.3% 4.9% 7.1% 6.1% 6.8% 7.4%
- Sensitivity
- <1 pg/mL
- Function
- Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 30931 MW
- Specificity
- Natural and recombinant mouse IL-1 beta
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin-1 beta
- Subcellular Location
- Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
- Protein Name
- Interleukin-1 beta
- Sample Data
- Concentration (pg/ml) 0 12.5 25 50 100 200 400 800 O.D. 0.019 0.091 0.174 0.334 0.675 1.111 1.763 2.52 Concentration (pg/ml) 0 12.5 25 50 100 200 400 800 O.D. 0.019 0.091 0.174 0.334 0.675 1.111 1.763 2.52 Concentration (pg/ml) 0 12.5 25 50 100 200 400 800 Concentration (pg/ml) 0 12.5 25 50 100 200 400 800 O.D. 0.019 0.091 0.174 0.334 0.675 1.111 1.763 2.52 O.D. 0.019 0.091 0.174 0.334 0.675 1.111 1.763 2.52
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
