- Catalog number
- EK0395
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin 1 receptor, type I (IL1R1), also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. IL1R1 along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. This protein is a receptor for interleukin 1 alpha (IL1A), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA) . It is an important mediator involved in many cytokine induced immune and inflammatory responses.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 188 179 191 173 182 7.15 3.9% Sample 2 973 977 935 964 962 16.42 1.7% Sample 3 4770 4715 5460 5318 5065 327.7 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 188 179 191 173 182 7.15 3.9% Sample 1 188 179 191 173 182 7.15 3.9% Sample 2 973 977 935 964 962 16.42 1.7% Sample 2 973 977 935 964 962 16.42 1.7% Sample 3 4770 4715 5460 5318 5065 327.7 6.4% Sample 3 4770 4715 5460 5318 5065 327.7 6.4%
- Synonyms
- Interleukin-1 receptor type 1; IL-1R-1; IL-1RT-1; IL-1RT1; CD121 antigen-like family member A; Interleukin-1 receptor alpha; IL-1R-alpha; Interleukin-1 receptor type I; p80; CD121a; Interleukin-1 receptor type 1, membrane form; mIL-1R1; mIL-1RI; Interleukin-1 receptor type 1, soluble form; sIL-1R1; sIL-1RI; IL1R1; IL1R, IL1RA, IL1RT1
- Gene Name
- IL1R1
- Gene ID
- 3554
- UniProt
- P14778
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: D21-T332
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Atherosclerosis, Cardiovascular, Cytokines, Hematopoietic Progenitors, Immunology, Innate Immunity, Interleukins, Lymphoid, Myeloid, Stem Cells, T Lymphocytic Lineage, Vascular Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 125 pg/mL - 8,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 188 973 4770 205 1005 4361 Standard deviation 8.46 63.24 195.57 10.86 77.38 248.57 CV (%) 4.5% 6.5% 4.1% 5.3% 7.7% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 188 973 4770 205 1005 4361 Standard deviation 8.46 63.24 195.57 10.86 77.38 248.57 CV (%) 4.5% 6.5% 4.1% 5.3% 7.7% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 188 973 4770 205 1005 4361 Mean (pg/ml) 188 973 4770 205 1005 4361 Standard deviation 8.46 63.24 195.57 10.86 77.38 248.57 Standard deviation 8.46 63.24 195.57 10.86 77.38 248.57 CV (%) 4.5% 6.5% 4.1% 5.3% 7.7% 5.7% CV (%) 4.5% 6.5% 4.1% 5.3% 7.7% 5.7%
- Sensitivity
- <10 pg/mL
- Function
- Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 65402 MW
- Specificity
- Natural and recombinant human IL1R1
- Gene Name Synonym
- Interleukin-1 receptor type 1
- Subcellular Location
- Membrane ; Single-pass type I membrane protein . Cell membrane . Secreted .
- Sequence Similarities
- Belongs to the interleukin-1 receptor family.
- Protein Name
- Interleukin-1 receptor type 1
- Sample Data
- Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.037 0.112 0.188 0.292 0.545 0.932 1.456 2.071 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.037 0.112 0.188 0.292 0.545 0.932 1.456 2.071 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.037 0.112 0.188 0.292 0.545 0.932 1.456 2.071 O.D. 0.037 0.112 0.188 0.292 0.545 0.932 1.456 2.071
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
