- Catalog number
- EK0397
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4. Human IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 29 28 30 29 1.11 3.8% Sample 2 158 160 151 163 158 4.41 2.7% Sample 3 484 469 488 480 480 7.08 1.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 29 28 30 29 1.11 3.8% Sample 1 31 29 28 30 29 1.11 3.8% Sample 2 158 160 151 163 158 4.41 2.7% Sample 2 158 160 151 163 158 4.41 2.7% Sample 3 484 469 488 480 480 7.08 1.4% Sample 3 484 469 488 480 480 7.08 1.4%
- Synonyms
- Interleukin-2; IL-2; T-cell growth factor; TCGF; Aldesleukin; IL2
- Gene Name
- IL2
- Gene ID
- 3558
- UniProt
- P60568
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A21-T153
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Cell Type Markers, Cytokines, Immunology, Innate Immunity, Interleukins, T Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 158 484 28 169 474 Standard deviation 1.27 8.84 30 1.14 10.98 38.39 CV (%) 4.1% 5.6% 6.2% 4.1% 6.5% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 158 484 28 169 474 Standard deviation 1.27 8.84 30 1.14 10.98 38.39 CV (%) 4.1% 5.6% 6.2% 4.1% 6.5% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 158 484 28 169 474 Mean (pg/ml) 31 158 484 28 169 474 Standard deviation 1.27 8.84 30 1.14 10.98 38.39 Standard deviation 1.27 8.84 30 1.14 10.98 38.39 CV (%) 4.1% 5.6% 6.2% 4.1% 6.5% 8.1% CV (%) 4.1% 5.6% 6.2% 4.1% 6.5% 8.1%
- Sensitivity
- <1 pg/mL
- Function
- Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 17628 MW
- Specificity
- Natural and recombinant human IL-2
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Interleukin-2
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the IL-2 family.
- Protein Name
- Interleukin-2
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.083 0.161 0.296 0.533 0.95 1.549 2.322 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.083 0.161 0.296 0.533 0.95 1.549 2.322 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.083 0.161 0.296 0.533 0.95 1.549 2.322 O.D. 0.008 0.083 0.161 0.296 0.533 0.95 1.549 2.322
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
