- Catalog number
- EK0399
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 62 61 60 63 61 1.11 1.8% Sample 2 243 247 277 267 258 14.02 5.4% Sample 3 1041 992 897 885 953 65.23 6.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 62 61 60 63 61 1.11 1.8% Sample 1 62 61 60 63 61 1.11 1.8% Sample 2 243 247 277 267 258 14.02 5.4% Sample 2 243 247 277 267 258 14.02 5.4% Sample 3 1041 992 897 885 953 65.23 6.8% Sample 3 1041 992 897 885 953 65.23 6.8%
- Synonyms
- Interleukin-2; IL-2; T-cell growth factor; TCGF; Il2; Il-2
- Gene Name
- IL2
- Gene ID
- 116562
- UniProt
- P17108
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A21-Q155
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Cell Type Markers, Cytokines, Immunology, Innate Immunity, Interleukins, T Cells
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 62 243 1041 56 254 1022 Standard deviation 4.71 15.06 53.09 4.81 17.52 71.54 CV (%) 7.6% 6.2% 7%% 8.6% 6.9% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 62 243 1041 56 254 1022 Standard deviation 4.71 15.06 53.09 4.81 17.52 71.54 CV (%) 7.6% 6.2% 7%% 8.6% 6.9% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 62 243 1041 56 254 1022 Mean (pg/ml) 62 243 1041 56 254 1022 Standard deviation 4.71 15.06 53.09 4.81 17.52 71.54 Standard deviation 4.71 15.06 53.09 4.81 17.52 71.54 CV (%) 7.6% 6.2% 7%% 8.6% 6.9% 7% CV (%) 7.6% 6.2% 7%% 8.6% 6.9% 7%
- Sensitivity
- <1 pg/mL
- Function
- Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 17633 MW
- Specificity
- Natural and recombinant rat IL-2
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Interleukin-2
- Subcellular Location
- Secreted.
- Protein Name
- Interleukin-2
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.053 0.116 0.171 0.286 0.478 0.792 1.467 2.454 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.053 0.116 0.171 0.286 0.478 0.792 1.467 2.454 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.053 0.116 0.171 0.286 0.478 0.792 1.467 2.454 O.D. 0.053 0.116 0.171 0.286 0.478 0.792 1.467 2.454
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
