- Catalog number
- EK0402
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3 (IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5. The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 37 33 37 35 2 5.7% Sample 2 205 240 222 228 223 12.61 5.6% Sample 3 387 460 404 393 411 28.93 7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 37 33 37 35 2 5.7% Sample 1 33 37 33 37 35 2 5.7% Sample 2 205 240 222 228 223 12.61 5.6% Sample 2 205 240 222 228 223 12.61 5.6% Sample 3 387 460 404 393 411 28.93 7% Sample 3 387 460 404 393 411 28.93 7%
- Synonyms
- Interleukin-3; IL-3; Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor; IL3
- Gene Name
- IL3
- Gene ID
- 3562
- UniProt
- P08700
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A20-F152
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Activated T-cells, mast cells, natural killer cells.
- Applications
- ELISA
- Field of Research
- Cytokines, Immunology, Innate Immunity, Interleukins
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 205 387 31 190 374 Standard deviation 1.68 9.63 21.736 1.73 8.93 28.42 CV (%) 7.6% 4.7% 7.2% 5.6% 4.7% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 205 387 31 190 374 Standard deviation 1.68 9.63 21.736 1.73 8.93 28.42 CV (%) 7.6% 4.7% 7.2% 5.6% 4.7% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 205 387 31 190 374 Mean (pg/ml) 33 205 387 31 190 374 Standard deviation 1.68 9.63 21.736 1.73 8.93 28.42 Standard deviation 1.68 9.63 21.736 1.73 8.93 28.42 CV (%) 7.6% 4.7% 7.2% 5.6% 4.7% 7.6% CV (%) 7.6% 4.7% 7.2% 5.6% 4.7% 7.6%
- Sensitivity
- <1 pg/mL
- Function
- Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 17233 MW
- Specificity
- Natural and recombinant human IL-3
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Interleukin-3
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the IL-3 family.
- Protein Name
- Interleukin-3
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.027 0.073 0.108 0.195 0.353 0.674 1.188 2.014 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.027 0.073 0.108 0.195 0.353 0.674 1.188 2.014 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.027 0.073 0.108 0.195 0.353 0.674 1.188 2.014 O.D. 0.027 0.073 0.108 0.195 0.353 0.674 1.188 2.014
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
