- Catalog number
- EK0404
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-ed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 31 34 34 33 1.5 4.5% Sample 2 216 213 203 197 207 7.62 3.6% Sample 3 508 515 448 502 493 26.52 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 31 34 34 33 1.5 4.5% Sample 1 35 31 34 34 33 1.5 4.5% Sample 2 216 213 203 197 207 7.62 3.6% Sample 2 216 213 203 197 207 7.62 3.6% Sample 3 508 515 448 502 493 26.52 5.3% Sample 3 508 515 448 502 493 26.52 5.3%
- Synonyms
- Interleukin-4 ; IL-4 ; B-cell stimulatory factor 1 ; Lymphocyte stimulatory factor 1 ; IL4 ; hCG_24113
- Gene Name
- IL4
- Gene ID
- 3565
- UniProt
- P05112
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: H25-S153
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 216 508 33 195 532 Standard deviation 2.03 12.52 21.33 2.04 11.89 29.26 CV (%) 5.8% 5.8% 4.2% 6.2% 6.1% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 216 508 33 195 532 Standard deviation 2.03 12.52 21.33 2.04 11.89 29.26 CV (%) 5.8% 5.8% 4.2% 6.2% 6.1% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 216 508 33 195 532 Mean (pg/ml) 35 216 508 33 195 532 Standard deviation 2.03 12.52 21.33 2.04 11.89 29.26 Standard deviation 2.03 12.52 21.33 2.04 11.89 29.26 CV (%) 5.8% 5.8% 4.2% 6.2% 6.1% 5.5% CV (%) 5.8% 5.8% 4.2% 6.2% 6.1% 5.5%
- Sensitivity
- <1.5 pg/mL
- Function
- Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 17492 MW
- Specificity
- Natural and recombinant human IL-4
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin-4
- Subcellular Location
- Secreted .
- Protein Name
- Interleukin-4
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.0 0.077 0.13 0.258 0.514 0.964 1.574 2.091 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.0 0.077 0.13 0.258 0.514 0.964 1.574 2.091 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.0 0.077 0.13 0.258 0.514 0.964 1.574 2.091 O.D. 0.0 0.077 0.13 0.258 0.514 0.964 1.574 2.091
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
