- Catalog number
- EK0405
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-ed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis.1 The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 15 14 13 16 14 1.11 7.9% Sample 2 96 90 93 94 93 2.16 2.3% Sample 3 280 285 256 248 267 15.61 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 15 14 13 16 14 1.11 7.9% Sample 1 15 14 13 16 14 1.11 7.9% Sample 2 96 90 93 94 93 2.16 2.3% Sample 2 96 90 93 94 93 2.16 2.3% Sample 3 280 285 256 248 267 15.61 5.8% Sample 3 280 285 256 248 267 15.61 5.8%
- Synonyms
- Interleukin-4; IL-4; B-cell IgG differentiation factor; B-cell growth factor 1; B-cell stimulatory factor 1; BSF-1; IGG1 induction factor; Lymphocyte stimulatory factor 1; Il4; Il-4
- Gene Name
- IL4
- Gene ID
- 16189
- UniProt
- P07750
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: H21-S140
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 7.8 pg/mL - 500 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 15 96 280 16 93 275 Standard deviation 1.11 6.62 20.44 1.48 6.69 20.9 CV (%) 7.4% 6.9% 7.3% 9.3% 7.2% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 15 96 280 16 93 275 Standard deviation 1.11 6.62 20.44 1.48 6.69 20.9 CV (%) 7.4% 6.9% 7.3% 9.3% 7.2% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 15 96 280 16 93 275 Mean (pg/ml) 15 96 280 16 93 275 Standard deviation 1.11 6.62 20.44 1.48 6.69 20.9 Standard deviation 1.11 6.62 20.44 1.48 6.69 20.9 CV (%) 7.4% 6.9% 7.3% 9.3% 7.2% 7.6% CV (%) 7.4% 6.9% 7.3% 9.3% 7.2% 7.6%
- Sensitivity
- <1 pg/mL
- Function
- Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 15834 MW
- Specificity
- Natural and recombinant mouse IL-4
- Gene Name Synonym
- Interleukin-4
- Subcellular Location
- Secreted.
- Protein Name
- Interleukin-4
- Sample Data
- Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.033 0.065 0.103 0.172 0.313 0.558 1.041 1.812 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.033 0.065 0.103 0.172 0.313 0.558 1.041 1.812 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.033 0.065 0.103 0.172 0.313 0.558 1.041 1.812 O.D. 0.033 0.065 0.103 0.172 0.313 0.558 1.041 1.812
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
